geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The hypotriglyceridemic effect of dietary n-3 FA is associated with increased beta-oxidation and reduced leptin expression


, : The hypotriglyceridemic effect of dietary n-3 FA is associated with increased beta-oxidation and reduced leptin expression. Lipids 38(10): 1023-1029

To study the mechanisms responsible for the hypotriglyceridemic effect of marine oils, we monitored the effects of high dietary intake of n-3 PUFA on hepatic and muscular beta-oxidation, plasma leptin concentration, leptin receptor gene expression, and in vivo insulin action. Two groups of male Wistar rats were fed either a high-fat diet [28% (w/w) of saturated fat] or a high-fat diet containing 10% n-3 PUFA and 18% saturated fat for 3 wk. The hypotriglyceridemic effect of n-3 PUFA was accompanied by increased hepatic oxidation of palmitoyl-CoA (125%, P < 0.005) and palmitoyl-L-carnitine (480%, P < 0.005). These findings were corroborated by raised carnitine palmitoyltransferase-2 activity (154%, P < 0.001) and mRNA levels (91%, P < 0.01) as well as by simultaneous elevation of hepatic peroxisomal acyl-CoA oxidase activity (144%, P < 0.01) and mRNA content (82%, P < 0.05). In contrast, hepatic carnitine palmitoyltransferase-1 activity remained unchanged despite a twofold increased mRNA level after n-3 PUFA feeding. Skeletal muscle FA oxidation was less affected by dietary n-3 PUFA, and the stimulatory effect was found only in peroxisomes. Dietary intake of n-3 PUFA was followed by increased acyl-CoA oxidase activity (48%, P < 0.05) and mRNA level (83%, P < 0.05) in skeletal muscle. The increased FA oxidation after n-3 PUFA supplementation of the high-fat diet was accompanied by lower plasma leptin concentration (-38%, P < 0.05) and leptin mRNA expression (-66%, P < 0.05) in retroperitoneal adipose tissue, and elevated hepatic mRNA level for the leptin receptor Ob-Ra (140%, P < 0.05). Supplementation of the high-fat diet with n-3 PUFA enhanced in vivo insulin sensitivity, as shown by normalization of the glucose infusion rate during euglycemic hyperinsulinemic clamp. Our results indicate that the hypotriglyceridemic effect of dietary n-3 PUFA is associated with stimulation of FA oxidation in the liver and to a smaller extent in skeletal muscle. This may ameliorate dyslipidemia, tissue lipid accumulation, and insulin action, in spite of decreased plasma leptin level and leptin mRNA in adipose tissue.

US$29.90

PMID: 14669966


Other references

Pope, R.P., 1999: Prevention of pelvic stress fractures in female army recruits. In 1991-1992, a pelvic stress fracture incidence of 11.2% was recorded in a cohort of 143 female Australian Army recruits. An incidence of 0.1% was recorded in a cohort of male recruits trained in the 1992-1993 year using a nearly identical progra...

Godynicki S.; Schwarz R.; Radke B., 1981: Micro morphological investigations of the rostral rete mirabile and cavernous sinus in sheep ovis aries. The thin walled vessels of the rete mirabile epidurale rostrale are invaginated into the sinus cavernosus. Because of that most rete vessels are not surrounded by connective or supporting tissues of organs, but only by blood. The fluid surrounding...

Morgan, S.R.; Boot Handford, R.P.; Sandle, G.I., 1999: Ca2+/calmodulin-mediated inhibition of human renal Na+ channels expressed in Xenopus oocytes. Journal of Physiology (Cambridge) 515P: 163P, Feb

Yates, P.J.; Nicholson, M.L., 2006: The aetiology and pathogenesis of chronic allograft nephropathy. Renal transplantation is the ultimate form of renal replacement therapy, and is the treatment of choice for many patients with end-stage renal failure. The advent of calcineurin inhibitor based immunosuppression resulted in the 1-year renal allogr...

Togna, A.Rita.; Latina, V.; Trefiletti, G.; Guiso, M.; Moschini, S.; Togna, G.I., 2014: 1-Phenil-6,7-dihydroxy-isochroman inhibits inflammatory activation of microglia. Inflammation plays a central role in the pathogenesis of several brain disorders and neuronal injury, and it develops as a consequence of glial cell activation. Activated microglial cells generate potentially damaging nitric oxide, oxygen free rad...

Jones, T.C.; Jennings, W.E., 1943: Equine influenza and related respiratory diseases in horses epizootiological and clinical observations. Respiratory diseases of horses, influenza, strangles, equine infectious pneumonia, and purpura hemorrhagica are discussed as they occurred in an outbreak at an Army Remount Depot involving 140 of 233 animals. Diagnoses were based on clinical sympt...

Anderson, S.G.; Ada, G.L., 1959: Some aspects of the reaction between crude Murray Valley encephalitis virus and deoxycholate. Crude MVE virus prepared from baby mouse brain was treated with deoxycholate (DCA) at 20[degree]C for 60 minutes. Concentrations of 0.03% DCA reduced virus titer by 2.5 log, and concentrations of 1.0% reduced infective virus titer 0.5 log but yiel...

Ziao, Z.Q., 1991: Immunohistochemical study of carcinoembryonic antigen (CEA) in non small cell carcinomas of the lung and bronchial epithelium adjacent to tumors. Immunoperoxidase staining for carcinoembryonic antigen (CEA) was performed on primary tumors and their metastatic lesions from 102 cases of the pulmonary non small cell carcinoma, and bronchial epithelium adjacent to 30 cases of tumors. Results in...

Mudde, A.H.; Bastiaanse, A.J.; Jonkers, H., 1987: TSH-IRMA as an initial diagnostic aid in suspected hyperthyroidism. Nederlands Tijdschrift Voor Geneeskunde 131(51): 2364-2368

Ibarra, J.; Downey, M.; Aguirre, A.; O'rourke, M.K.; Lebowitz, M., 2000: Yuma asthma study. American Journal of Epidemiology 151(11 Supplement): S80, June 1