+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The identification of foam-forming soluble proteins from wheat (Triticum aestivum) dough

, : The identification of foam-forming soluble proteins from wheat (Triticum aestivum) dough. Proteomics 5(6): 1612-1623

Proteomic methods have been used to identify foam-forming soluble proteins from dough that may play an important role in stabilising gas bubbles in dough, and hence influence the crumb structure of bread. Proteins from a soluble fraction of dough (dough liquor) or dough liquor foam have been separated by two-dimensional gel electrophoresis, and 42 identified using a combination of matrix-assisted laser desorption/ionization-time of flight and quadrupole-time of flight analyses. Major polypeptide components included beta -amylase, tritin and serpins, with members of the alpha -amylase/trypsin inhibitor family being particularly abundant. Neither prolamin seed storage proteins nor the surface-active protein puroindoline were found. Commonly used dough ingredients (NaCl, Na L-ascorbate) had only a minor effect on the 2-DE protein profiles of dough liquor, of which one of the more significant was the loss of 9 kDa nonspecific lipid transfer protein. Many proteins were lost in dough liquor foam, particularly tritin, whilst a number of alpha -amylase inhibitors were more dominant, suggesting that these are amongst the most strongly surface-active proteins in dough liquor. Such proteins may play a role determining the ability of the aqueous phase of doughs, as represented by dough liquor, to form an elastic interface lining the bubbles, and hence maintain their integrity during dough proving.


PMID: 15789342

DOI: 10.1002/pmic.200401035

Other references

Peters, Robert W., 1960: Research on psychological parameters of sound. Experimental approaches to the determination of psychological parameters for complex sounds are discussed and relevant studies are summarised. The results of a series of experiments which were conducted treating the problem of parameters for compl...

Kaplan, J.; Ward, D.M.; D.D.menico, I., 2011: The molecular basis of iron overload disorders and iron-linked anemias. Iron homeostasis in vertebrates requires coordination between cells that export iron into plasma and those that utilize or store plasma iron. The coordination of iron acquisition and utilization is mediated by the interaction of the peptide hormon...

Oliveira, M.C. de; Carvalho, I.D., 2002: Yield and lesions on carcass of broilers raised on different litters and under different stocking densities. The total meat production, carcass yield and lesions on breast, hocks and foot pads of the carcass of 488 broilers raised in two litter types and three stocking densities were evaluated. The experimental design was entirely randomized in an arrang...

Singh, B.; Champlain, J., 1972: Altered ontogenesis of central noradrenergic neurons following neonatal treatment with 6-hydroxydopamine. Brain Research 48: 432-437

Hendry, J.H.; Booth, C.; Potten, C.S., 2002: Endothelial cells and radiation gastrointestinal syndrome. Science 294(5546): 1411-1411

Storer, R.J.mes; Pozo-Rosich, P.; Goadsby, P.J., 2007: Glycine receptors modulate neurotransmission in the trigeminocervical complex of the rat. Cephalalgia 27(10): 1183

Parmar, M.; Scott, R.P., 1996: Mivacurium chloride and late onset congenital myopathy. We describe the successful use of the short-acting, non-depolarizing neuromuscular blocking agent, mivacurium, in a 53-yr-old female patient with late onset congenital myopathy, undergoing elective submucous resection of the inferior turbinates. S...

Zettler, F.W.; Abo-El-Nil, M.M., 1977: Bean yellow mosaic virus infections of gladiolus in florida. Bean yellow mosaic virus (BYMV) isolates from Florida[USA]-grown gladiolus [Gladiolus .times. grandiflorus] were compared with BYMV isolates collected elsewhere from gladiolus and other hosts. All gladiolus isolates induced similar symptoms in pea...

De-Woody, J.A.drew; Chesser, R., K.; Baker, R., J., 1999: A translocated mitochondrial cytochrome b pseudogene in voles (Rodentia: Microtus). A full-length cytochrome b pseudogene was found in rodents; it has apparently been translocated from a mitochondrion to the nuclear genome in the subfamily Arvicolinae. The pseudogene (psi cytb) differed from its mitochondrial counterpart at 201 o...

Okamoto, M.; Hojo, Y.; Inoue, K.; Matsui, T.; Kawato, S.; McEwen, B.S.; Soya, H., 2012: Mild exercise increases dihydrotestosterone in hippocampus providing evidence for androgenic mediation of neurogenesis. Mild exercise activates hippocampal neurons through the glutamatergic pathway and also promotes adult hippocampal neurogenesis (AHN). We hypothesized that such exercise could enhance local androgen synthesis and cause AHN because hippocampal stero...