+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods

, : The identification of isoprenoids that bind in the intersubunit cavity of Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase by complementary biophysical methods. Acta Crystallographica. Section D, Biological Crystallography 61(Pt 1): 45-52

The discovery of a distinct metabolic pathway, the non-mevalonate or 1-deoxy-D-xylulose-5-phosphate ( DOXP) pathway for isoprenoid precursor biosynthesis, in eubacteria and apicomplexan parasites has revealed a new set of potential drug targets. The emphasis of research on this pathway has been on delineating the intermediates and the biochemical and structural characterization of component enzymes. Two new monoclinic crystal forms of recombinant Escherichia coli 2C-methyl-D-erythritol-2,4-cyclodiphosphate ( MECP) synthase cocrystallized with (i) CMP and (ii) CMP and MECP show well defined electron density at the subunit interface suggestive of an isoprenoid-like ligand. 31P NMR analysis of the recombinant protein sample indicates the presence of bound diphosphate species and electrospray mass spectrometry identifies a mixture of isopentenyl diphosphate (and/or dimethylallyl diphosphate), geranyl diphosphate and farnesyl diphosphate in an approximate ratio of 1: 4: 2. The most prevalent species, geranyl diphosphate, was successfully modelled into the electron density, revealing the important protein - ligand interactions that stabilize binding of the isoprenoid. The observation that MECP synthase binds three metabolites that are produced by enzymes two, three and four stages downstream in isoprenoid biosynthesis suggests that feedback regulation of the non-mevalonate pathway is possible.


PMID: 15608374

DOI: 10.1107/S0907444904025971

Other references

Herold, T.Rike.; Rist, K.; Priglinger, S.Georg.; Ulbig, M.Werner.; Wolf, A., 2016: Long-term results and recurrence rates after spironolactone treatment in non-resolving central serous chorio-retinopathy (CSCR). To evaluate the long-term results of spironolactone in non-resolving central serous chorio-retinopathy (CSCR) and recurrence rates of CSCR. Interventional uncontrolled open-label prospective clinical trial of patients with non-resolving CSCR who w...

Moravec, F.; Nie, P.; Scholz, T.; Wang, G., 2003: Some trematodes and cestodes of fishes mainly from Hubei Province, central China. The present paper comprises a systematic survey of trematodes and cestodes based on helminthological examinations of 176 specimens of 22 species of freshwater fishes, belonging to 11 families of 6 fish orders, from central China (mostly from lakes...

Moore, S., 1996: I have heard that nurse practitioners are cost-effective providers of ambulatory care. Journal of Emergency Nursing: Jen 22(2): 145-146

Braun, H.J., 1961: Spring movement of water in trees and scions.. Observations on Betula verrucosa, Populus Sect. Aigeiros, Acer pseudoplatanus, Quercus robur and Fraxinus excelsior led to the following conclusions. In the stem, movement of water begins in the middle of March and goes on until flushing. In the f...

Azizi-Zohan, A.; Kamgar-Haghighi, A.A.; Sepaskhah, A.R., 2008: Crop and pan coefficients for saffron in a semi-arid region of Iran. Saffron (Crocus sativus L.) is an important prennial crop planted in Iran. The growing period of saffron is mostly winter and spring, which makes it a popular crop in areas with limited water supply. Crop coefficients, K-c (the ratio of crop poten...

Singh A.; Agarwal R.L., 1990: Effect of wheat mustard mixtures and nitrogen on yield and economics of wheat. Indian Journal of Agronomy 35(3): 326-327

Zhai, L.; Li, D.; Ding, Y., 1991: Establishment of a field management system with coupling cotton bollworm (Heliothis armigera) dynamics and cotton plant development. Yingyong Shengtai Xuebao, 22: 134-140

Garcia, G.A.; Soldi-Lose, Héloïse.; Nahon, L.; Powis, I., 2009: Photoelectron circular dichroism spectroscopy in an orbitally congested system: the terpene endoborneol. We have measured the photoelectron circular dichroism (PECD) of single enantiomers of endoborneol in the photon region from 9.9 to 23.6 eV by combining circularly polarized synchrotron radiation and a velocity map imaging technique. A photoelectro...

Turchetto, E.; Infante, R.; Sechi, A.M., 1955: Effect of thyroxin on lipotropic activity of pantothenic acid. Bollettino Della Societa Italiana di Biologia Sperimentale 31(3-4): 233-234

Gaba, D.; Cooper, J., 2003: Landmark report published on patient safety. Journal of Clinical Monitoring and Computing 16(3): 231-232