geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions


, : The identity of proteins associated with a small heat shock protein during heat stress in vivo indicates that these chaperones protect a wide range of cellular functions. Journal of Biological Chemistry 279(9): 7566-7575

The small heat shock proteins (sHSPs) are a ubiquitous class of ATP-independent chaperones believed to prevent irreversible protein aggregation and to facilitate subsequent protein renaturation in cooperation with ATP-dependent chaperones. Although sHSP chaperone activity has been studied extensively in vitro, understanding the mechanism of sHSP function requires identification of proteins that are sHSP substrates in vivo. We have used both immunoprecipitation and affinity chromatography to recover 42 proteins that specifically interact with Synechocystis Hsp16.6 in vivo during heat treatment. These proteins can all be released from Hsp16.6 by the ATP-dependent activity of DnaK and co-chaperones and are heat-labile. Thirteen of the putative substrate proteins were identified by mass spectrometry and reveal the potential for sHSPs to protect cellular functions as diverse as transcription, translation, cell signaling, and secondary metabolism. One of the putative substrates, serine esterase, was purified and tested directly for interaction with purified Hsp16.6. Hsp16.6 effectively formed soluble complexes with serine esterase in a heat-dependent fashion, thereby preventing formation of insoluble serine esterase aggregates. These data offer critical insights into the characteristics of native sHSP substrates and extend and provide in vivo support for the chaperone model of sHSP function.

US$19.90

PMID: 14662763

DOI: 10.1074/jbc.M310684200


Other references

Vallejo M.A., 1990: The placebo effect in the biofeedback treatment of headache. Pain (SUPPL 5): S50

Shiferaw, W.; Shelton, H.M.; So, H.B., 1992: Tolerance of some subtropical pasture legumes to waterlogging. The waterlogging tolerance of 8 pasture legumes was studied in a glasshouse at the University of Queensland, Brisbane. Australia during the spring of 1989. The species were Lotus pendunculatus cv. Maku and accessions G4704 and 67677, L. corniculat...

Lee C.; Harvey H.H., 1986: Localization of aluminum in tissues of fish. Water Air & Soil Pollution 30(3-4): 649-656

Rey, D.; Marigo, G.; Pautou, M.P.ule, 1996: Phenolic compounds of Alnus glutinosa and control of larval populations of Culicidae. This work examines the hypothesis that phenolic compounds occur in the regulation of fauna found in Aedes habitats. The litter of these habitats is composed mainly of Alnus glutinosa leaves which release tannins and other phenolic compounds during...

Rapoport, J.L.; Ryland, D.H.; Kriete, M., 1992: Drug treatment of canine acral lick. An animal model of obsessive-compulsive disorder. Canine acral lick dermatitis is a naturally occurring disorder in which excessive licking of paws or flank can produce ulcers and infection that require medical treatment. Forty-two dogs with severe chronic canine acral lick dermatitis were treate...

Dall'Osto, L.; Cazzaniga, S.; Wada, M.; Bassi, R., 2014: On the origin of a slowly reversible fluorescence decay component in the Arabidopsis npq4 mutant. Over-excitation of photosynthetic apparatus causing photoinhibition is counteracted by non-photochemical quenching (NPQ) of chlorophyll fluorescence, dissipating excess absorbed energy into heat. The PsbS protein plays a key role in this process,...

Goncharov, A.A.; Komarov, V.I.; Shevchuk, N.O., 1983: Device for checking the quality of teatcup liners. The device comprises a head with a vacuum gauge and a pending stem with a central vacuum channel provided with radial apertures at a point about halfway down its length. For testing, the device is pushed into the liner, and a vacuum is applied thr...

Micali, S.; Sighinolfi, M.C.; Celia, A.; D.S.efani, S.; Grande, M.; Cicero, A.F.; Bianchi, G., 2006: Can Phyllanthus niruri affect the efficacy of extracorporeal shock wave lithotripsy for renal stones? A randomized, prospective, long-term study. Purpose: Phyllanthus niruri is a plant used in Brazilian folk medicine for the treatment of urolithiasis. We assessed the efficacy of P. niruri after extracorporeal shock wave lithotripsy for renal stones. Materials and Methods: We prospectively e...

Leubner-Metzger, G.F.undt, C.M.ins, F.Jr, 1996: Effects of gibberellins, darkness and osmotica on endosperm rupture and class I beta-1,3-glucanase induction in tobacco seed germination. The "Havana 425" cultivar of Nicotiana tabacum L. is photodormant. Gibberellins (e.g. 10(-5) M GA4 or GA7) can substitute for light in releasing dormancy. Measurements of beta-1,3-glucanase activity, mRNA accumulation and the activity of...

Fujimura, Y.; Tachibana, H.; Yamada, K., 2004: Lipid raft-associated catechin suppresses the FcepsilonRI expression by inhibiting phosphorylation of the extracellular signal-regulated kinase1/2. The major green tea catechin, (-)-epigallocatechin-3-O-gallate (EGCG), has a suppressive effect on the expression of the high-affinity IgE receptor FcepsilonRI, which is key molecule in the IgE-mediated allergic reactions. Here we show that EGCG b...