+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The immunohistochemical assessment of hypoxia, vascularity and proliferation in bladder carcinoma

, : The immunohistochemical assessment of hypoxia, vascularity and proliferation in bladder carcinoma. RadioTherapy and Oncology 72(2): 159-168

Background and purpose: Hypoxia and proliferation are important determinants of radiation responsiveness; prospective measures of these before radiotherapy may enable individualisation of treatment schedules. Immunohistochemical techniques offer a potential means of achieving this in routine biopsy material. Material and methods: Cellular hypoxia as measured by pimonidazole fixation and immunohistochemistry has been evaluated in a series of human bladder cancers with dual staining of sections for pimonidazole and either the vascular markers, CD31/34, or proliferation markers, Ki-67 or cyclin A. Twenty one tumour specimens were examined suitable for the double staining technique. Results: The median hypoxic fraction was 9% (range 0-38). Seven tumours did not stain for pimonidazole and 11 exhibited necrosis. The mean vascular density ranged from 16.7 to 160.6 vessels per mm2. The median hot spot count was 30 (range 16-43). There was a statistically significant increase in vessel density in hypoxic compared to oxic regions measured by both vessel density (P = 0.02) and hot spot count (P = 0.004). Proliferation indices decreased from oxic to hypoxic areas close to blood vessels. Conclusions: We have demonstrated that bladder cancer exhibits a range of hypoxia, proliferation and vascular density which may be used to form the basis for patient selection for hypoxia modification, accelerated radiotherapy and vascular targeting agents.


PMID: 15297134

DOI: 10.1016/j.radonc.2004.03.017

Other references

Reynolds, A.; McFaul, A.; Goddard, E.W., 1991: Testing for causality between advertising expenditures and Canadian demand for cheese and butter. Using quarterly data for the period 1977-87, the notion of Granger causality is employed to test the causal relationship existing between advertising and sales of cheese and butter in Canada. Results suggest that advertising expenditures influence...

Bartak, Miroslav., 1986: The Czechoslovak species of Lonchopteridae Diptera Ceskoslovenske druhy celedi Lonchopteridae Diptera. Dipterologica Bohemoslovaca, 4: 61-69

Julia, O.; Comas, J.; Vives Rego, J., 2000: Second-order functions are the simplest correlations between flow cytometric light scatter and bacterial diameter. Second-order mathematical relationships between bacterial cell diameter determined by electric particle analyser and flow cytometric forward light scatter in axenic cultures are obtained and discussed. Since it is technically impossible today to o...

Baik, B.K.; Paszczynska, B.; Czuchajowska, Z., 1997: Does HWS wheat produce good quality noodles independent of growing conditions?. Cereal Foods World 42(8): 666

Webster, J.; Dan., 1993: The manubrium-sternum bridge in songbirds (Oscines). Proceedings of the Indiana Academy of Science. 1992( ) June 1; 1013-4: 299-308

Cowan, I.; McT.C.rl, G.C., 1945: The Northern Elephant Seal (Mirounga angustirostris) in British Columbia waters and vicinity. Canadian Field Naturalist, 595: 170-171

Cleophas, T.J.; de Jong, S.J.; Niemeyer, M.G.; Tavenier, P.; Zwinderman, K.; Kuypers, C., 1993: Changes in life-style in men under sixty years of age before and after acute myocardial infarction: a case-control study. A case-control study was conducted to examine the presence of psychosocial factors before, during, and after acute myocardial infarction (AMI) in Dutch men under sixty years of age. The study included 42 AMI patients and 48 individuals in an age-a...

Kennerson, M.L.; Zhu, D.; Walizada, G.; Nicholson, G.A., 2003: Transcriptional map of the dominant intermediate Charcot-Marie-Tooth neuropathy locus on chromosome 19p12-p132. American Journal of Human Genetics 73(5): 482, November

Kay, R.H., 1965: Light-stimulated and light-inhibited bio-luminescence of the euphausiid Meganyctiphanes norvegica (G. O. Sars). Proceedings of the Royal Society, 162B 365-386

Pokorny, S.B.; Jason, L.A.; Schoeny, M.; Curie, C.J.; Townsend, S.M., 2001: Eliminating invalid self-report survey data. A sample of 6,370 students in Grades 6 to 8 completed a questionnaire on their attitudes and use of alcohol, tobacco, and other drugs. A subsample showed questionable data based on three criteria: missing responses, invalid responses, and inconsis...