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The impact of LysfwdarwArg surface mutations on the crystallization of the globular domain of RhoGDI


, : The impact of LysfwdarwArg surface mutations on the crystallization of the globular domain of RhoGDI. Acta Crystallographica Section D Biological Crystallography 60(2): 275-280, February

The potential of rational surface mutagenesis for enhanced protein crystallization is being probed in an ongoing effort. In previous work, it was hypothesized that residues with high conformational entropy such as Glu and Lys are suitable targets for surface mutagenesis, as they are rarely incorporated in crystal contacts or protein-protein interfaces. Previous experiments using LysfwdarwAla, GlufwdarwAla and GlufwdarwAsp mutants confirmed that mutated proteins were more likely to crystallize. In the present paper, the usefulness of LysfwdarwArg mutations is studied. Several mutations of the globular domain of human RhoGDI were generated, including the single mutants K105R, K113R, K127R, K138R and K141R, the double mutants K(98,99)R and K(199,200)R and the triple mutants K(98,99,105)R and K(135,138,141)R. It is shown that LysfwdarwArg mutants are more likely to crystallize than the wild-type protein, although not as likely as LysfwdarwAla mutants. Out of the nine mutants tested, five produced diffracting crystals, including the K(199,200)R double mutant, which crystallized in a new space group and exceeded by apprx1.0 ANG the resolution of the diffraction of the wild-type crystal. Major crystal contacts in the new lattice were created by the mutated epitope.

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Sriramachandramurthy, N.; Venkateswaralu, P.; Ramarao, Y., 1983: A promising chilli strain with a marker gene. The Capsicum annuum strain X235 was developed by crossing a mutant having yellow anthers with the normal strain G4 and selecting plants with G4 characteristics plus yellow anthers in the F2 and subsequent generations. X235 gave higher yields than...

Urbani, E., 1964: Proteases And Morphogenesis. The behavior of endocellular proteolytic enzymes in morphogenesis is interesting with regard to the relations which may exist between proteases and cellular differentiation phenomena. The article reports the results obtained by the author and cowo...

Li, C-Ke.; Xu, S-Yuan.; Chen, L-Xue., 2002: Sources and elimination of interference in patch clamp electrophysiological experiment. The sources of the interference in the patch clamp electrophysiological experiments were investigated, and the methods for eliminating the interference, along with the authors experience, are introduced.

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Helms, C.A.; Kaban, L.B.; McNeill, C.; Dodson, T., 1989: Temporomandibular joint: morphology and signal intensity characteristics of the disk at MR imaging. Magnetic resonance (MR) imaging has been used in the temporomandibular joint (TMJ) primarily to define the disk position. This report examines altered morphology and signal intensity characteristics of the TMJ disk as they relate to the severity o...

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Batchelor, J.M.; Handfield-Jones, S.E., 2011: Lesson of the Week. When a cyst is not a cyst. Bmj 342: D2844-D2844

Stirban, M.; Tara, G.; Voicu, G., 1972: The influence of the soil fertilization system on photosynthesis and pigmentation in vine rootstocks. The soil contents of N, P and K after each fertilizer treatment were compared with an unfertilized control. The differences were attributed to differential ion accumulation from the fertilizers. The effects of the ionic equilibrium of the soil on...

Wu, T.H.ng; Tsai, C.Y.uh; Yu, C.L., 2001: Cyclooxygenase expression, modulated by proinflammatory cytokines or lipopolysaccharide in rat glomerular mesangial cells. Journal of the American Society of Nephrology 12(Program and Abstract Issue): 667A, September