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The impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from Yersinia pseudotuberculosis

, : The impact of abiotic factors (temperature and glucose) on physicochemical properties of lipids from Yersinia pseudotuberculosis. Biochimie 86(12): 875-881

The impact of the availability of glucose in nutrition medium and growth temperature on the composition and thermotropic behavior of lipids from Yersinia pseudotuberculosis (Enterobacteriaceae) was studied. Y. pseudotuberculosis was grown in nutrition broth (NB) with/without glucose at 8 and 37 degreeC, corresponding to the temperatures of saprophytic and parasitic phases of this bacterium life. The decrease of phosphatidylethanotamine, phosphatidylglycerol and unsaturated fatty acids and the parallel increase of lysophosphatidylethanolamine and diphosphatidylglycerol and saturated and cyclopropane acids were the most significant changes with temperature in bacterial phospholipid (PL) classes and fatty acids, respectively. Glucose did not effect the direction of temperature-induced changes in the contents of PLs, fatty acids, however it enhanced (for PLs) or diminished (for fatty acids) intensity of these changes. The thermally induced transitions of lipids were studied by differential scanning calorimetry (DSC). It was revealed that the addition of glucose to NB induced a sharp shift of DSC thermograms to lower temperatures in the "warm" variants of bacteria. The peak maximum temperature (Tmax) of thermal transitions dropped from 50 to 26 degreeC that is the optimal growth temperature of Y pseudotuberculosis. Tmax of total lipids of the cells grown at 8 degreeC without glucose in NB was equal to growth temperature that corresponded to the classical mechanism of homeoviscous adaptation of bacteria. An addition of glucose to NB at this growth temperature caused the subsequent reduction of Tmax to -8 degreeC, while the temperature ranges of thermograms were not substantially changed. So, not only the temperature growth of bacteria, but also the presence of glucose in NB can modify the physical state of lipids from Y pseudotuberculosis. In this case, both factors affect additively. It is suggested that glucose influences some membrane-associated proteins and then the fluidity of lipid matrix through temperature-inducible genes. Copyright 2004 Elsevier SAS. All rights reserved.


PMID: 15667937

DOI: 10.1016/j.biochi.2004.10.011

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Morero, B.; Rodriguez, Mía.B.; Campanella, E.A., 2016: Environmental impact assessment as a complement of life cycle assessment. Case study: Upgrading of biogas. This work presents a comparison between an environmental impact assessment (EIA) and a life cycle assessment (LCA) using a case study: upgrading of biogas. The upgrading of biogas is studied using three solvents: water, physical solvent and amine....

Kovacs, J.; Kovacs, A., 1977: Membrane alterations in the seminal vesicle cells of the mouse during cooling in vitro. Cooling of mouse seminal vesicle cells in vitro induced detachment of ribosomes from certain areas of the rough endoplasmic reticulum and folding of the resulting smooth membranes around parts of the cytoplasm according to electron micrographs. Li...

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Prill, H., 1966: Die vogelweit des Naturschutzgebietes 'Grosser Schwerin.'. Falke, 13: 269-276

Tsai, S-Jen.; Liao, D-Lieh.; Yu, Y.W-Y.; Chen, T-Jui.; Wu, H-Chi.; Lin, C-Hui.; Cheng, C-Ya.; Hong, C-Jee., 2005: A study of the association of (Val66Met) polymorphism in the brain-derived neurotrophic factor gene with alcohol dependence and extreme violence in Chinese males. From studies of genetic-knockout animals, brain-derived neurotrophic factor (BDNF), a member of the neurotrophin growth-factor family, has been implicated in both alcohol preference and aggressive behaviour. To test whether a BDNF genetic variant...

Slivitskiĭ, M.G., 1970: Absorption of volatile fatty acid salt in the abomasum in sheep. Fiziolohichnyi Zhurnal 16(3): 374-378

Esashi, T.; Hanai, M., 1993: Bioavailability of magnesium contained in purple laver (Asakusa-Nori) by rats with scarce magnesium, being evaluated from serum magnesium, kidney calcification, and bone magnesium contents. The availability of magnesium in the alga purple laver (Asakusa nori; Porphyra tenera) was evaluated in Mg-restricted, 4-week-old male rats after 3 weeks feeding with 4 different diets: control (20SC); Mg-restricted (-Mg20SC); -Mg20SC plus purple...

Cattani, S.; Galassi, C., 2001: Database of air pollution and air quality indicators for the principal Italian cities: the ITARIA-WHO project. This paper presents air quality data collected in eight Italian cities (Turin, Genoa, Milan, Bologna, Florence, Rome, Naples, Palermo) during the period 1994-98. Storage and analysis of data have been performed by the ITARIA collaborative group in...

Stark, C.J.; Stepans, M.Beth.Flanders., 2007: A comparison of blood pressure in term, low birth-weight infants of smoking and nonsmoking mothers. Infants exposed to the components of cigarette smoke in utero are at an increased risk for perinatal death, low birth weight, sudden infant death syndrome, and premature delivery. The purpose of this pilot study was to compare blood pressure value...

Pincus, J.H., 1969: Carbon dioxide fixation in rat brain: relationship to cerebral excitability. Experimental Neurology 24(3): 339-347