+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The impact of excitotoxic blockade on the evolution of injury following combined mechanical and hypoxic insults in primary rat neuronal culture

, : The impact of excitotoxic blockade on the evolution of injury following combined mechanical and hypoxic insults in primary rat neuronal culture. Neurobiology of Disease 17(3): 378-384

Traumatic brain injury (TBI) involves alterations in neuronal physiology, often complicated by secondary hypoxic or hypotensive events. Excitotoxicity is an important process induced in both TBI and hypoxic or ischemic insults to the brain. We investigated two hypotheses: (1) excitotoxicity is more prominent following combined mechanical and hypoxic injury than either alone; (2) both AMPA and NNMA receptor activation mediate combined mechanical and hypoxic injury. Media in primary mixed neuronal cultures were replaced with conditioned media containing MK801 (NMDA antagonist) and/or NBQX (AMPA/kainate antagonist). Cultures were then subjected to mechanical injury. Afterward, media were exchanged for hypoxic media containing the antagonist, and plates were placed in hypoxia chambers for 7 h. At 24 h following hypoxia, LDH release, trypan blue uptake, and morphologic changes were assessed. Blockade had no effect after mechanical injury. After hypoxia, MK801 and combined MK801/NBQX decreased LDH and trypan blue to control levels. NBQX alone after hypoxia had less impact. After combined mechanical injury and hypoxia, both MK801 and NBQX partially reduced LDH and trypan blue. Combining the antagonists led to reduction to control values for both endpoints. We conclude that excitotoxic processes are more prominent after combined than isolated injuries in neurons and that increased cell death is mediated by both NMDA and AMPA receptor activation following combined injuries. Copyright 2004 Elsevier Inc. All rights reserved.


PMID: 15571974

DOI: 10.1016/j.nbd.2004.07.017

Other references

Fan, H.; Hu, Q-Da.; Xu, F-Jian.; Liang, W-Quan.; Tang, G-Ping.; Yang, W-Tai., 2012: In vivo treatment of tumors using host-guest conjugated nanoparticles functionalized with doxorubicin and therapeutic gene pTRAIL. The combination of gene therapy and chemotherapy may increase the therapeutic efficacy in the treatment of patients. In this work, the anti-cancer drug Dox and therapeutic gene pTRAIL-loaded host-guest co-delivery system was assayed for the possib...

Buccheri G.; Capretto G.; D.D.nato V.; Esposito P.; Ferruzza G.; Pescatore T.; Russo Ermolli E.; Senatore M.R.; Sprovieri M.; Bertoldo M.; Carella D.; Madonia G., 2002: A high resolution record of the last deglaciation in the southern Tyrrhenian Sea; environmental and climatic evolution. A multidisciplinary study was carried out on core C106 collected in the Salerno Gulf (southern Tyrrhenian Sea). Two tephra layers recovered in the core, delta (super 18) O record of Globigerinoides ruber and Globigerina bulloides and seven (super...

Hinz, R., 1975: Description of ichneumon nebulosae new species hymenoptera ichneumonidae. Ichneumon nebulosae .male.female. is described and compared to I. languidus Wesmael.

Parkington, H.C.; Tare, M.; Tonta, M.A.; Coleman, H.A., 1993: Stretch revealed three components in the hyperpolarization of guinea-pig coronary artery in response to acetylcholine. 1. Membrane potential was recorded with intracellular microelectrodes from the smooth muscle of coronary arteries of guinea-pigs, and the responses to endothelium-derived relaxants were studied under a variety of conditions. 2. Stimulation of the...

Bisque Ramon E., 1963: Challenge of mineral exploration. Mines Magazine 53(7): 14

Rupp, J.C.; Woolhouse, M.E., 1999: Impact of geographical origin on mating behaviour in two species of Biomphalaria (Planorbidae: Gastropoda). Studies of inbreeding and outcrossing have traditionally concentrated on matings within populations. The influence of geographical origin on mate choice in animals from different populations has received less attention. We investigated whether pla...

Cerny, V., 1987: Acariosis of fish bred in aquaria. Ziva, 353: 109

Sposito Garrison, 1990: Molecular models of iron adsorption on mineral surfaces. Reviews in Mineralogy: ages 261-279. 1990.

Kim, C-Hoon.; Kim, M., 2009: Defining Reported Errors on Web-based Reporting System Using ICPS From Nine Units in a Korean University Hospital. The aim of the present study was to delineate and evaluate the feasibility of a web-based error reporting system using the International Classification of Patient Safety (ICPS) in a Korean university hospital. Seventy-five staff nurses were encour...

Sherman, P-M.; Hassall, E.; Fagundes-Neto, U.; Gold, B-D.; Kato, S.; Koletzko, S.; Orenstein, S.; Rudolph, C.; Vakil, N.; Vandenplas, Y., 2011: A global, evidence-based consensus on the definition of gastroesophageal reflux disease in the pediatric population. Archives de Pediatrie 17(11): 1586-1593