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The influence of N- and O-glycosylation inhibitors on the glycosylation profile of cellular membrane proteins and adhesive properties of carcinoma cell lines

, : The influence of N- and O-glycosylation inhibitors on the glycosylation profile of cellular membrane proteins and adhesive properties of carcinoma cell lines. International Journal of Molecular Medicine 17(4): 669-674

The effects of N- and O-glycosylation inhibitors on the expression of membrane proteins (MUC1 and some integrins) were evaluated in human endometrial (Ishikawa) and breast (MCF-7) cancer cells. Subconfluent cells were treated with 1-3 mg% concentration of tunicamycin and 2-10 mM of benzyl-N-acetyl-alpha-galactosaminide for 1-2 days, and used for flow cytometry, immunohistochemical stainina, adhesion test and Western blotting.


PMID: 16525726

Other references

Robin Cecile; Guillocheau Francois; L.S.lleuz Antoine; Bechennec Francois; Gorican Spela, 2001: Turbiditic systems of the Hawasina Basin (Upper Permian-Upper Cretaceous); geodynamic evolution of the Tethys margin in Oman. ASF Association des Sedimentologistes Francais 36: 307

Beaussart, M., 1960: Myoclonic encephalopathy of the infant with hypsarrhythmia. E.E.G. study before and after ACTH treatment. Revue Neurologique 103: 243-250

Thijsse J.T., 1957: Scientific hydrology. International Union of Geodesy and Geophysics General Assembly = Union Geodesique et Geophysique Internationale Comptes Rendus de la

Yoshino, T.; Cao, L.; Nishiuchi, R.; Matsuo, Y.; Yamadori, I.; Kondo, E.; Teramoto, N.; Hayashi, K.; Takahashi, K.; Kamikawaji, N., 1995: Ligation of HLA class II molecules promotes sensitivity to CD95 (Fas antigen, APO-1)-mediated apoptosis. CD95 (Fas antigen/APO- 1) is up-regulated in activated lymphocytes, and monoclonal antibody (mAb) to CD95 induces apoptosis. HLA class II molecules play a key role in antigen presentation, ligation of which induces signal transduction. We examined...

Zhang,; Schulte, F.; Bulfone Paus, S.; Hoffmann, F., 2000: The effect of corticosteroid and cyclosporin A on murine corneal allograft rejection. Background: The immunomodulatory T-helper type 1 (Th1) cytokine interferon-gamma (IFN-gamma) was measured in serum and cornea to ascertain its general contribution to corneal graft rejection and to establish a rational basis for the decision for o...

de Baere, T., 1998: Re: Diagnosis and management of ureteroiliac artery fistula: value of provocative arteriography followed by common iliac artery embolization and extraanatomic arterial bypass grafting. Journal of Urology 159(6): 2102-2103

Lopez-Mejia, I.Cristina.; D.T.ledo, M.; Della Seta, F.; Fafet, P.; Rebouissou, C.; Deleuze, V.; Blanchard, J.Marie.; Jorgensen, C.; Tazi, J.; Vignais, M-Luce., 2014: Tissue-specific and SRSF1-dependent splicing of fibronectin, a matrix protein that controls host cell invasion. Cell invasion targets specific tissues in physiological placental implantation and pathological metastasis, which raises questions about how this process is controlled. We compare dermis and endometrium capacities to support trophoblast invasion,...

Hubble, L.M., 1978: Comparability and equivalence of estimates of IQS from Revised Beta Examination and Wechsler Adult Intelligence Scale among older male delinquents. Comparability and equivalence of IQ estimates based on responses to the Revised Beta Examination and to the Wechsler Adult Intelligence Scale [WAIS] were assessed for 63 predominantly white older male delinquents in a state correctional facility....

Plummer, Julie A., 1997: Reproductive biology of Zantedeschia aethiopica. Scott, J K [Editor], Wykes, B J [Editor] Proceedings of a Workshop on Arum Lily (Zantedeschia aethiopica) 7-9

Didenko, A.; Merkulova, O.S., 1966: Changes in the SH-group and RNA content of motor neurons of the cat spine during stimulation of the posterior radix by means of electrical impulses of varying frequency. A sector of the spinal column about 5 cm in length was exposed in the anaesthetized cat. The posterior radix was electrically stimulated at the L7 and S1 levels. Impulses (0.2 msec) were delivered for 20 min. Two minutes after the initial impulse,...