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Transient transfection of porcine granulosa cells after 3D culture in barium alginate capsules


, : Transient transfection of porcine granulosa cells after 3D culture in barium alginate capsules. International Journal of Immunopathology and Pharmacology 18(4): 677-681

Three-dimensional culture systems in barium alginate capsules can be employed to maintain primary granulosa cells in an undifferentiated state for almost 6 days. This is due to a self-organization of cells in a pseudofollicular structure. The transfection of primary granulosa cells is a necessary condition when employing these culture systems for several purposes, for example as an in vitro toxicity test or the development of oocytes or zygotes.

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