+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Washing of cord blood grafts after thawing: high cell recovery using an automated and closed system

, : Washing of cord blood grafts after thawing: high cell recovery using an automated and closed system. Vox Sanguinis 87(3): 165-172

Background and Objectives Cord blood (CB) progenitor cells are an alternative source of haematopoietic stem cells for bone marrow reconstitution. The critical importance of cell dose in the clinical outcome has motivated the need to develop techniques aimed at reducing cell losses and increasing reproducibility. This aim of this study was to evaluate an automated CB washing protocol of thawed cord blood units using the Sepax device. Materials and Methods After an initial 1 : 1 dilution using a dextran/albumin-containing buffer, the cells were washed in order to obtain a final product ready for transplantation. The automatic method was compared with the conventional manual washing procedure. Blood samples were taken after thawing and after washing. The processing time, viability and mean recovery of nucleated cells (TNC) and progenitors were determined. Results The automatic procedure resulted in a median recovery of 93% CD34+ cells and 89% TNC; no significant differences were observed between methods. In addition, median viability, as assessed by annexin V and 7-aminoactinomycin D (7-AAD), was 98% and 94%, respectively, within CD34+ cells. Conclusions The automatic washing method described is as effective as the manual method in terms of viability and progenitor cells recovery, but faster and easier for the operators to perform. Overall, our data suggest that the automatic method is safe and suitable for the routine washing of thawed CB grafts in the clinic.


PMID: 15569068

DOI: 10.1111/j.1423-0410.2004.00550.x

Other references

Rollins, G., 2004: Breaking the language barrier. Helping hands. Trustee 57(7): 30, 32-30, 32

Bosco, L.; Peroni, A.; Schena, D.; Colato, C.; Girolomoni, G., 2011: Cutaneous manifestations of Churg-Strauss syndrome: report of two cases and review of the literature. Churg-Strauss syndrome (CSS) is a small-vessel vasculitis characterized by severe asthma, lung/tissues infiltrates, extravascular necrotizing granulomas, and eosinophilia. Cutaneous involvement is common but may not be highly suggestive. Two typic...

Schonhaut B, L.; Pérez R, M.; Castilla F, A.María.; Castro M, S.; Salinas A, P.; Armijo R, Ián., 2017: Predictive value of Ages & Stages Questionnaires for cognitive performance at early years of schooling. The Ages and Stages questionnaires (ASQ) has been recently validated in our country for developmental screening. The objective of this study is evaluate the validity of ASQ to predict low cognitive performance in the early years of schooling. Diag...

Ipsen, R.; Olsen, K.; Skibsted, L.H.; Qvist, K.B., 2002: Gelation of whey protein induced by high pressure. Whey protein concentrates (WPC) and purified beta -lactoglobulin ( beta -Lg) were subjected to high hydrostatic pressure (300 MPa for 30 min) and the resulting gels evaluated with respect to gel strength and non-incorporated liquid (NIL). Gel stre...

Dos Santos, Violantino, 1934: Sobre os parasitas do sistema nervoso que denomino Neuroplasma viannae e Neuroplasma caviae. In the brain of a hen which died of diphtheria the author found a parasite in the form of 2 cysts of 43 X 38 [mu], with a thin membrane containing many spores 1.5 [mu] long, fusiform, and having a central nucleus. In the brain of guinea pigs cysts...

Pasteur, I.P.; Tronko, N.D., 1996: The effects of thyroid-stimulating hormone on the iodide transport in the newborn porcine thyroid cells in primary culture. Journal of Endocrinology 148(SUPPL ): P217

Yun, Q.; He, Y-Bing.; Lv, W.; Zhao, Y.; Li, B.; Kang, F.; Yang, Q-Hong., 2016: Chemical Dealloying Derived 3D Porous Current Collector for Li Metal Anodes. A 3D porous Cu current collector is fabricated through chemical dealloying from a commerial Cu-Zn alloy tape. The interlinked porous framework naturally integrated can accommodate Li deposition, suppressing dendrite growth and alleviating the huge...

Serrao, J.E.uardo; Cruz Landim, C.D., 1996: The ultrastructure of the pyloric valve region in bees, with considerations of fluid flux in the digestive tract. The pyloric valve region of the gut was studied in adult bees. The pyloric valve is formed by folds of the hindgut epithelium being covered by a thin cuticle (1.7 to 2.0 pm). This cuticle has spine-like structures at the proximal end of the valve...

Purtilo, D.T.; Kersey, J.H.; Hallgren, H.M.; Fox, K.R.; Yunis, E.J., 1973: Alpha-fetoprotein: diagnostic and prognostic use in patients with hepatomas. American Journal of Clinical Pathology 59(3): 295-299

Anonymous, 2008: Falsified data in " Effects of whey peptides on cardiovascular disease risk factors" (J Clin Hypertens [Greenwich] 2006;8(11):775-782)--JJ Pins, JM Keenan. Journal of Clinical Hypertension 10(8): 631-631