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cis-Dimer formation of E-cadherin is independent of cell-cell adhesion assembly in vivo

, : cis-Dimer formation of E-cadherin is independent of cell-cell adhesion assembly in vivo. Biochemical and Biophysical Research Communications 316(3): 822-826

Cadherin-based cell-cell adhesions play important roles in embryonic development and in the maintenance of normal tissue architecture. Little is known, however, about the mechanisms of controlling cadherin dynamics at the cell surface. We previously demonstrated that E-cadherin functions as a cis (lateral)-dimer on the cell surface by chemical cross-linking. In this study, we examined the dynamics of E-cadherin cis-dimer formation during cell-cell adhesion assembly by using chemical cross-linking. Although treatment with cytochalasin D, a potent inhibitor of actin polymerization, was shown to inhibit the formation of cell-cell contacts, the dynamics of E-cadherin cis-dimer formation was not affected. This result indicated that the cis-dimer formation procedure is independent of cell-cell adhesion assembly in vivo. However, the cell aggregation and dissociation assays showed that the cytochalasin D treatment shifted the cadherin-based cell adhesion from a strong to a weak state. Taken together, these results strongly support the possibility that the E-cadherin cis-dimer is a minimal functional unit in cadherin-mediated cell-cell adhesion in vivo.


PMID: 15033474

DOI: 10.1016/j.bbrc.2004.02.123

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Benisty, J.; Roller, M.; Sahar, G.; Paz, R.; Vidne, B.; Sagie, A., 2000: Iatrogenic left ventricular-right atrial fistula following mitral valve replacement and tricuspid annuloplasty: diagnosis by transthoracic and transesophageal echocardiography. Acquired left ventricle-to-right atrium communications are a known complication of valvular heart surgery. Previous reports have described the clinical features and diagnosis using cardiac catheterization. We report two cases of acquired left vent...

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Bhattacharyya S.C., 1968: Contents of medicinal plants selinum vaginatum d. Deutsche Apotheker Zeitung 108(32): 1161

Ancrenaz, M.; Lackman Ancrenaz, I.; Mundy, N., 1994: Field observations of aye-ayes in Madagascar. Data are presented from a field study of aye-ayes (Daubentonia madagascariensis) in an area of degraded secondary forest in northeast Madagascar. Animals were followed by radiotelemetry for 3 months during the cool dry season, when productivity of...

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Nyman, M.; Bonhomme, Fçois.; Alam, T.M.; Rodriguez, M.A.; Cherry, B.R.; Krumhansl, J.L.; Nenoff, T.M.; Sattler, A.M., 2002: A general synthetic procedure for heteropolyniobates. The heteropolyanions of W, Mo, and V, which have found numerous applications, are formed simply by acidification of solutions of their oxoanions. Under similar conditions, these oxoanion precursors are not available for Nb, and Nb-oxo chemistry is...

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