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Expression site silencing and life-cycle progression appear normal in Argonaute1-deficient Trypanosoma brucei

, : Expression site silencing and life-cycle progression appear normal in Argonaute1-deficient Trypanosoma brucei. Molecular and Biochemical Parasitology 149(1): 102-107

An experiment was conducted to investigate whether ablation of Argonaute1 (AGO1) in bloodstream forms (BS) of Trypanosoma brucei was associated with changes in the levels of high molecular weight retrosposon transcripts and retrosposon-derived small interfering RNAs (siRNAs). RNA from Lister 427 ago1-/- cells were analysed with probes specific for the ubiquitous INGI or the site-specific SLACS retroelements.


PMID: 16735068

DOI: 10.1016/j.molbiopara.2006.04.005

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