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Alloxan is an inhibitor of O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase


, : Alloxan is an inhibitor of O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase. Biochemical and Biophysical Research Communications 350(4): 1038-1043

We have previously shown that streptozotocin (STZ) inhibits O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase (O-GlcNAcase), the enzyme that removes O-GlcNAc from proteins. In light of this observation, we explored the possibility that the diabetogenic toxin alloxan, an O-GlcNAc transferase (OGT) inhibitor, might also inhibit O-GlcNAcase. Alloxan inhibited islet O-GlcNAcase with a dose-response much like that of STZ. Similar to STZ, islet O-GlcNAcase was more susceptible to alloxan inhibition than was brain O-GlcNAcase. Alloxan directly inhibited recombinant O-GlcNAcase activity with a dose-response very similar to that of STZ. Subsequent LC/MS/MS analysis revealed that alloxan modified the tryptic digest pattern of the enzyme. One tryptic peptide LGCFEIAK(894-901) was modified by alloxan. Two other tryptic peptides, LDQVSQFGCR(158-167) and SFALLFDDIDHNMCAADK(168-185), both N-terminal active site peptides, were absent after alloxan treatment. Together, these data demonstrate that alloxan is an inhibitor of O-GlcNAc-selective N-acetyl-beta-D-glucosaminidase, with inhibition corresponding to an altered tryptic digest pattern of N-terminal active site peptides.

US$19.90

PMID: 17045574

DOI: 10.1016/j.bbrc.2006.09.155


Other references

Mandelbaum, M.Helena.Sant.'ana., 1980: Sterilization control. Role of the nurse. The author describes methods and techniques of sterilization control and emphatizes its importance in the control of infection.

Tarver, E.; Morales, M.F., 1951: The reaction between actomyosin and various nucleotides and phosphates as followed by ultraviolet absorption. Journal of Cellular Physiology 37(2): 235-246

Smith, N.J., 1976: Gaining and losing weight in athletics. Participants in many sports, such as wrestling, gymnastics, and light-weight crew, attempt to reduce body weight to achieve a maximum ratio of muscle strength to body weight. Such weight reduction should result only from reduction in excess body f...

Safe, S., 2015: MicroRNA-Specificity Protein (Sp) Transcription Factor Interactions and Significance in Carcinogenesis. NlmCategory="UNASSIGNED">Specificity protein (Sp) transcription factors (TFs) such as Sp1, Sp3 and Sp4 are overexpressed in tumors and Sp1 is a negative prognostic factor for multiple tumor types. Sp TFs regulate expression of pro-onc...

Wiethop, Dave, 1999: Crazy for coir. American Vegetable Grower 47(4): 3*

Hawkins, M.; Scarsbrook, A.F.; Pavlitchouk, S.; Moore, N.R.; Bradley, K.M., 2007: Detection of an occult thymoma on 99Tcm-Tetrofosmin myocardial scintigraphy. Thymoma is an uncommon tumour, which is often asymptomatic and may be detected coincidentally on cross-sectional imaging performed for other reasons. We report a case of thymoma detected on myocardial scintigraphy performed for suspected coronary...

Anonymous, 2011: Stanford engineers redefine how the brain plans movement. New neurological measurement technologies and algorithms are allowing researchers a more complete look into how the brain functions. Engineers at Stanford are using these tools to better understand how the brain prepares to initiate a body motion...

Spijkerman, A.M.W.; Dekker, J.M.; D.V.gt, F.; Ruige, J.B.; Stehouwer, C.D.A.; Nijpels, G.; Bouter, L.M.; Heine, R.J., 1999: Leptin level is not associated with cardiovascular mortality The Hoorn Study. Diabetologia 42(SUPPL 1): A106, Aug

Yamamoto, T.; Ito, K.; Suzuki, K.; Yamanaka, H.; Ebihara, K.; Sasaki, A., 2002: Rapidly progressive malignant epithelioid angiomyolipoma of the kidney. Journal of Urology 168(1): 190-191

Snitsarev, V.; Whiteis, C.A.; Abboud, F.M.; Chapleau, M.W., 2002: Does baroreceptor resetting occur in isolated baroreceptor neurons in culture?. After periods of acute hypertension, the action potentials (APs) of baroreceptor (BR) afferents are suppressed and the pressure-activity curve is shifted to higher pressure. This BR "resetting" maintains the ability of BRs to buffer fluc...