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Apheresis products of the Amicus (TM) and the AS.TEC 204 (R) cell separators are comparable with regard to dendritic cells derived from the mononuclear cell collection


, : Apheresis products of the Amicus (TM) and the AS.TEC 204 (R) cell separators are comparable with regard to dendritic cells derived from the mononuclear cell collection. Vox Sanguinis 92(1): 37-41

Background In this study, we investigated the quality of autologous mononuclear cells (MNC) collected with two different cell separators using standard MNC-apheresis procedure modalities. MNCs were purified by density gradient centrifugation and cultured according to standard protocols to generate dendritic cells (DC) and 1 x 10(7)/ml immature DCs were pulsed with tumour lysate for 3 days and subsequently characterized by fluorescent-activated cell sorter analysis.Results No difference was found in the monocyte content of either apheresis product (P = 0.07) and in the overall yield of MNCs (P = 0.7). Mature DCs as defined by their phenotype revealed also no significant difference: Amicus', 118 x 10(6) cells +/- 91 vs. AS.TEC 204 (R), 128 x 10(6) cells +/- 137 (P = 0.55), respectively, although the contamination with platelets (threefold) and red cells (twofold) was significantly higher in the AS.TEC 204 group (P < 0.05) than in the Amicus group.Conclusion The Amicus and the AS.TEC 204 are equally capable in providing MNCs for the generation of DCs and the amount of concomitantly collected red cells and platelets had no impact on the final DC yield.

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Barrientos Y.; Izturiz A.; Urbani F., 2005: Characterization of a cyanobacterial mat; composition, cover, and mineral assemblages associated with the AR-5 Springs, Hacienda El Chupadero, Aragua State. Geos (Caracas) 38: 112

Asano, K.; Yoshimura, S.; Nakane, A., 2014: Alteration of intestinal microbiota in mice orally administered with salmon cartilage proteoglycan, a prophylactic agent. Proteoglycan (PG) extracted from salmon nasal cartilage has potential to be a prophylactic agent. Daily oral administration of the PG attenuates systemic inflammatory response in the experimental mouse models. In this study, we applied the culture...

DiTullio, Giacomo R., 1996: Dimethylsulfide concentrations in the southern Ross Sea during austral summer 1995-1996. Antarctic Journal of the United States, 312: 127-128 Review

Shin, J.Sook.; Kang, K.Ah.; Kim, E.Sil.; Zhang, R.; Piao, M.Jing.; Ko, D.Ok.; Wang, Z.Hong.; Maeng, Y.Hee.; Eun, S.Yong.; Chae, S.; Chung, H.Sook.; Hyun, J.Won., 2008: Cytoprotective activity of annphenone against oxidative stress-induced apoptosis in V79-4 lung fibroblast cells. We have elucidated the cytoprotective effect of annphenone (2,4-dihyroxy-6-methoxy-acetophenone 4-<i>O</i>- beta -D-glucopyranoside) against oxidative stress-induced apoptosis. Annphenone scavenged intracellular reactive oxygen species...

Sacks L.E.; Macgregor J.T., 1981: The bacillus subtilis multi gene mutagen screening test detection of mutagens to which the standard ames salmonella tester strains are insensitive. Environmental Mutagenesis 3(3): 337

Gozhik, P.F., 1964: Find of fossil fauna remains of Anthropogene brackish water molluscs in the Prus Valley. Dopovidi Akademiyi Nauk Ukrayinskoyi SSR: (1): 100-102

Storey, KB.; Storey, JM., 1989: Comment les animaux survivent au gel. Recherche (Paris): 20: 332-341 No. 208

Victorov, Sergey V., 2008: Remote sensing and GIS for coastal zone. Invited lecture covers several problems of Remote Sensing applications on Coastal Zone. Coastal Zone includes waters and adjacent shores of coastal lands as well as islands, transitional and intertidal areas, salt marshes, wetlands, and beaches. D...

Nordestgaard A., 1990: Chervil for seed production. In the period of 1983-1989 different trials and examinations on chervil for seed production sown in the autumn were carried out at the Government research stations. Examinations on sowing depth and trials on sowing time, seed rate, nitrogen rate i...

Gudkova, E.I.; Krasil'nikov, A.P., 1994: Method for assessment and characteristics of sensitivity of bacteria to disinfectants. Klinicheskaya Laboratornaya Diagnostika 0(6): 48-50