geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Characterization of an alternative splice variant of human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): a possible modulator of nucleotidase activity and purinergic signaling


, : Characterization of an alternative splice variant of human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): a possible modulator of nucleotidase activity and purinergic signaling. Archives of Biochemistry and Biophysics 457(1): 7-15

Nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) is a cell surface, membrane-bound enzyme that hydrolyzes extracellular nucleotides, thereby modulating purinergic signaling. An alternatively spliced variant of NTPDase3 was obtained and analyzed. This alternatively spliced variant, termed "NTPDase3 beta", is produced through the use of an alternative terminal exon (exon 11) in place of the terminal exon (exon 12) in the full-length NTPDase3, now termed "NTPDase3 alpha". This results in an expressed protein lacking the C-terminal cytoplasmic sequence, the C-terminal transmembrane helix, and apyrase conserved region 5. The cDNA encoding this truncated splice variant was detected in a human lung library by PCR. Like the full-length NTPDase3 alpha, the alternatively spliced NTPDase3 beta was expressed in COS cells after transfection, but only the full-length NTPDase3 alpha is enzymatically active and properly trafficked to the plasma membrane. However, when the truncated NTPDase3 beta was co-transfected with full-length NTPDase3 alpha, there was a significant reduction in the amount of NTPDase3 alpha that was properly processed and trafficked to the plasma membrane as active enzyme, indicating that the truncated form interferes with normal biosynthetic processing of the full-length enzyme. This suggests a role for the NTPDase3 beta variant in the regulation of NTPDase3 nucleotidase activity, and therefore the control of purinergic signaling, in those cells and tissues expressing both NTPDase3 alpha and NTPDase3 beta.

US$19.90

PMID: 17126282

DOI: 10.1016/j.abb.2006.10.013


Other references

Kamal, A.; Sinha, A.; Hutfless, S.M.; Afghani, E.; Faghih, M.; Khashab, M.A.; Lennon, A.Marie.; Yadav, D.; Makary, M.A.; Andersen, D.K.; Kalloo, A.N.; Singh, V.K., 2016: Hospital admission volume does not impact the in-hospital mortality of acute pancreatitis. Multiple factors influence mortality in Acute Pancreatitis (AP). To evaluate the association of demographic, clinical, and hospital factors with the in-hospital mortality of AP using a population-based administrative database. The Maryland HSCRC d...

Who, 1996: Environmental Health Criteria, 177 1,2-Dibromoethane. This text is a comprehensive, highly detailed, investigation of the environmental behavior and toxicity of 1,2-dibromoethane. This chemical has limited usage as a gasoline additive and as a soil fumigant. Over 300 published reports are examined. C...

Conde-Salazar, L.; Romero, L.; Guimaraens, D.; Harto, A., 1982: Contact dermatitis in an oil painter. Contact Dermatitis 8(3): 209-210

Hosking, J.R.M.; Wallis, J.R., 1993: Some statistics useful in regional frequency analysis. Regional frequency analysis uses data from a number of measuring sites to estimate the frequency distribution of the observed data at each site. A region is defined as a group of sites each of which has data drawn from the same frequency distribut...

Churchill, D.R.; Persinger, M.A.; Thomas, A.W., 1994: Geophysical variables and behavior: LXXVII. Increased geomagnetic activity and decreased pleasantness of spontaneous narratives for percipients but not agents. A total of 48 adults were tested in 24 separate pairs either during the morning (10:00 A.M.) or evening (10:00 P.M.). One member of each pair was instructed to record (write) impressions and descriptions of 8 different art pieces (5 min. each). At...

Przybilski, M.; Deb, R.; Erdmann, D.; Germann, G., 2004: New developments in skin replacement materials. Current treatment strategies in intensive care medicine permit survival of patients with burns of more than 80% of the total body surface area (TBSA). Major burns result in extensive skin defects. Thus, burn victims often suffer from scar contract...

Graner, J.L., 1988: Against a total ban on smoking in our hospitals. Pharos of Alpha Omega Alpha-Honor Medical Society. Alpha Omega Alpha 51(3): 14-15

Colrain, I.M.; Taylor, J.; Mclean, S.; Buttery, R.; Wise, G.; Montgomery, I., 1993: Dose dependent effects of alcohol on visual evoked potentials. The effects of various alcohol doses on components of the visual evoked potential were investigated. Using a repeated measures, Latin square design, five alcohol dose conditions were administered to ten male subjects: 0.00 (placebo); 0.28; 0.36; 0...

Wilkening R.B., 1985: Adaptation of the fetal pulmonary circulation to an increase in oxygen partial pressure following fetal acidemia and maternal oxygen administration. American Review Of Respiratory Disease Suppl: A254

Silva, J.M.N.; Cadima, J.F.C.L.; Pereira, J.M.C.; Gregoire, J.M., 2004: Assessing the feasibility of a global model for multi-temporal burned area mapping using SPOT-VEGETATION data. The possibility of using the Systeme Probatoire de l'Observation de la Terre (SPOT)-VEGETATION (VGT) data for global burned area mapping with a single algorithm was investigated. Using VGT images from south-eastern Africa (Mozambique, Zimbabw...