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Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue


, : Analysis of type I and IV collagens by FT-IR spectroscopy and imaging for a molecular investigation of skeletal muscle connective tissue. Analytical and Bioanalytical Chemistry 386(7-8): 1961-1966

Many muscular diseases result from abnormal organization of connective tissue and/or collagen network formation. Only a few molecular imaging techniques are able to analyze this collagen network by differentiating collagen types. In this study, FT-IR spectroscopy was used to analyze type I and IV collagens, the most important compounds of which are perimysium and endomysium, respectively. Secondary structure of collagen types was determined by curve-fitting the 1,700-1,480 cm(-1) spectral interval. Type I collagen could be differentiated from type IV by its higher amounts of triple helix and alpha-helix, but lower amounts of beta-sheets (P < 0.01). FT-IR imaging was then used to determine structural features of perimysium and endomysium collagen network in bovine Flexor carpi radialis muscle. Secondary structure of proteins contained in perimysium and endomysium was found to be very close to type I and IV collagens, respectively. FT-IR spectroscopy and imaging are thus analytical tools that might be used for investigating biodistribution and assembly of collagen types in connective tissues.

US$19.90

PMID: 17043797

DOI: 10.1007/s00216-006-0828-0


Other references

Wyzlic, I.M.; Tjarks, W.; Soloway, A.H.; Anisuzzaman, A.K.M.; Rong, F.G.ang; Barth, R.F., 1994: Strategies for the design and synthesis of boronated nucleic acid and protein components as potential delivery agents for neutron capture therapy. Purpose: Strategies for the design and synthesis of boronated nucleosides, amino acids, and peptides as potential delivery agents for boron neutron capture therapy (BNCT) are described. Methods and Materials: For BNCT to be a useful treatment moda...

Pompeiano, O.; Carli, G.; Kawamura, H., 1967: Transmission of sensory information through ascending spinal hindlimb pathways during sleep and wakeful-ness. The trans-mission of somatic afferent volleys through ascending spinal hind-limb pathways was investigated during physiological sleep and wake-fulness in unrestrained, unanesthetized cats. A late mass discharge was recorded from the ventral quadra...

Kattan, Gustavo H., 1998: Impact of brood parasitism. Why do house wrens accept shiny cowbird eggs?. Oxford Ornithology Series 9: 212-220

Lorion, J.; Duperron, S.; Gros, O.; Cruaud, C.; Samadi, S., 2009: Several deep-sea mussels and their associated symbionts are able to live both on wood and on whale falls. Bathymodiolin mussels occur at hydrothermal vents and cold seeps, where they thrive thanks to symbiotic associations with chemotrophic bacteria. Closely related genera Idas and Adipicola are associated with organic falls, ecosystems that have been...

Morey, R.; Schertz, C., 1983: Collection, processing and combustion of corncobs to provide heat for drying corn Biomass fuel. 3rd annual Solar and Biomass Workshop April 26-28-1983 Holiday Inn Atlanta Airport North Atlanta Georgia co sponsors United States Department of Agriculture et al: 232

Chakravarthy, B.; Anderson, C.L.; Ludlow, J.; Lotfipour, S.; Vaca, F.E., 2010: The relationship of pedestrian injuries to socioeconomic characteristics in a large Southern California County. The goal of this study is to explore the relationship between pedestrian injuries and socioeconomic characteristics. Pedestrian collisions were identified in the data of the California Statewide Integrated Traffic Records System (SWITRS), which is...

Klemetsdal, G.; Reitan, K.; Osteras, O., 1992: Relationship between udder exterior and mastitis. During 3 seasons milk samples from Norwegian Red cows of a high and a low milk yield line were analysed by CMT or Charm test. Teat length, diam. and distances were measured, and teat shape and extension, teat tip shape, and leakage were studied. H...

Ter-Avanesyan, M.; Inge-Vechtomov, S.; Surguchev, A.; Smirnov, V., 1982: Relationship of mitochondrial and cytoplasmic protein synthesis in the yeast Saccharomyces cerevisiae. Doklady: Biochemistry Akademiia nauk SSSR 264(1-6): 169-172

Ediriweera, S.A., 1976: Colles fractures and fracture - separation of the lower radial epiphysis. Ceylon Medical Journal 21(1): 47-51

Sheehan, D.M.; Young, M., 1979: Diethylstilbestrol and estradiol binding to serum albumin and pregnancy plasma of rat and human. The equilibrium binding of diethylstilbestrol (DES) and 17 beta-estradiol (E2) to plasma proteins has been characterized. DES exhibits a 10- to 20-fold greater binding affinity index for bovine serum albumin and rat plasma than E2. As expected, E2...