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Endogenous brain-derived neurotrophic factor triggers fast calcium transients at synapses in developing dendrites


, : Endogenous brain-derived neurotrophic factor triggers fast calcium transients at synapses in developing dendrites. Journal of Neuroscience 27(5): 1097-1105

Brain-derived neurotrophic factor (BDNF) is involved in many aspects of the formation of functional neuronal networks. BDNF signaling regulates neuronal development not only globally, at the level of entire neurons or networks, but also at a subcellular level and with high temporal specificity; however, the spatiotemporal characteristics of intrinsic BDNF signaling are essentially unknown. Here, we used calcium imaging to directly observe intrinsic BDNF signaling in developing hippocampal neurons. We found that blocking intrinsic BDNF signaling with function-blocking BDNF antibodies (alpha BDNF) or K252-a reduced the frequency of spontaneously occurring fast and localized calcium rises in dendrites. Conversely, focal application of BDNF evoked fast and local dendritic calcium transients, which required activation of TrkB (tropomyosin-related kinase B) receptors as well as activation of voltage-gated sodium and calcium channels. Virus-mediated expression of PSD-95: CFP (postsynaptic density-95 tagged with cyan fluorescent protein) revealed that spontaneous local calcium transients occurred frequently at postsynaptic sites along the dendrite. The frequency of synaptically localized calcium transients was specifically reduced by blocking intrinsic BDNF signaling, whereas nonsynaptic calcium rises were not affected. Furthermore, focal BDNF delivery evoked localized and fast calcium elevations specifically at postsynaptic sites. Together, our results demonstrate that BDNF-dependent calcium signaling in developing hippocampal neurons is fast and occurs at synapses. These temporal and spatial characteristics of intrinsic BDNF signaling as well as its relative abundance renders BDNF an ideal signaling molecule in the establishment of specific synaptic connectivity and functional neuronal networks.

US$19.90

PMID: 17267564

DOI: 10.1523/JNEUROSCI.3590-06.2007


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Peng, Z.; Li, L-Zhen.; Zhao, M-Jun., 2012: Two new apterous species of Lathrobium Gravenhorst (Coleoptera, Staphylinidae, Paederinae) from Fujian, East China. Two new apterous species of the genus Lathrobium Gravenhorst, 1802 from Fujian Province, East China, Lathrobium daocongchaoi Peng & Li, sp. n. and Lathrobium fujianense Peng & Li,sp. n., are described and illustrated.

Steyn, D.G., 1933: Factors concerned in the determination of toxicity of plants. The following factors are discussed: In the plant,[long dash]nature of soil and cultivation, climatic conditions, nature and intensity of light, season, stage of development, toxic parts, state of plant, transmission of degree of toxicity to proge...

Anonymous, 1977: Charles A. Black: Edward W. Browning Award. Agronomy Journal: 69 (1) 135

Pesek J.; Peskova J., 1993: Prospects of mining and depletion of coal reserves in the Czech Republic. Uhli 41(4): 136-138

Silvestre, R.; Brandao, C.R.berto Ferreira., 2000: The ants Hymenoptera, Formicidae attracted to baits in a cerrado island in Cajuru county, state of Sao Paulo, Brazil Formigas Hymenoptera, Formicidae atraidas a iscas em uma ilha de cerrado no municipio de Cajuru, Estado de Sao Paulo, Brasil. The richness and the diversity of the ant fauna attracted to baits in a regenerated Cerrado "island" (21deg. 18-27' S, 47deg. 12-20' W) were studied based on a quantitative survey in six collecting events, carried out every two...

Phillips, N., 1994: The use of zeta potential in food processing applications. Abstracts of Papers American Chemical Society 208(1-2): AGFD 152

Shashkanov V.A.; Kosterov A.A., 1998: Artificial magnetite-bearing sediments with different initial magnetic states of magnetic particles; field dependencies of their orientational magnetization and anisotropy. Geologica Carpathica (Bratislava): , Pages 229. 1998.

Wamicha W.N., 1984: A geo-pedological model for the Nguu Volcano area (Wilaya ya Machakos, Kenya); its importance in the mapping of Quaternary surficial deposits. International Geological Congress, Abstracts = Congres Geologique International, Resumes: , Pages 424. 1984.

Dunsky, I.L.; Fife, W.A.; Richey, E.O., 1973: Determination of revised Air Force permissible exposure levels for laser radiation. American Industrial Hygiene Association Journal 34(6): 235-240

Zierz, S.; Engel, A.G., 1987: Are there two forms of carnitine palmitoyltransferase in muscle?. Mitochondria were isolated from rat skeletal muscle, heart, and liver and from human skeletal muscle. The distribution of CPT I and CPT II was studied by measuring CPT activity and malonyl-CoA sensitivity before and after disruption of the mitocho...