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Independent evolution of pyrimethamine resistance in Plasmodium falciparum isolates in Melanesia


, : Independent evolution of pyrimethamine resistance in Plasmodium falciparum isolates in Melanesia. Antimicrobial Agents and ChemoTherapy 51(3): 1071-1077

Pyrimethamine resistance in Plasmodium falciparum has previously been shown to have emerged once in Southeast Asia, from where it spread to Africa. Pyrimethamine resistance in this parasite is known to be conferred by mutations in the gene encoding dihydrofolate reductase (dhfr). We have analyzed polymorphisms in dhfr as well as microsatellite haplotypes flanking this gene in a total of 285 isolates from different regions of Melanesia (Papua New Guinea, Vanuatu, and the Solomon Islands) and Southeast Asia (Thailand and Cambodia). Nearly all isolates (92%) in Melanesia were shown to carry a dhft double mutation (CNRNI [underlining indicates the mutation]) at positions 50, 51, 59, 108, and 164, whereas 98% of Southeast Asian isolates were either triple (CIRNI) or quadruple (CIRNL) mutants. Microsatellite analysis revealed two distinct lineages of dhft double mutants in Melanesia. One lineage had the same microsatellite haplotype as that previously reported for Southeast Asia and Africa, suggesting the spread of this allele to Melanesia from Southeast Asia. The other lineage had a unique, previously undescribed microsatellite haplotype, indicative of the de novo emergence of pyrimethamine resistance in Melanesia.

US$19.90

PMID: 17210777

DOI: 10.1128/AAC.01186-06


Other references

Kim, E.Edmund., 2003: Advances in Nuclear Oncology. Cancer Research and Treatment 35(4): 281-288

Platz, E.A.; Michaud, D.S.; Willett, W.C.; Giovannucci, E., 2002: Interrelation of energy intake and body size with metastatic/fatal prostate cancer. Proceedings of the American Association for Cancer Research Annual Meeting 43: 933-934, March

Greenberg, M.H.; Hill, R.M., 1973: The physiology of contact lens imprints. American Journal of Optometry and Archives of American Academy of Optometry 50(9): 699-702

Lebourg, L.; Solas, J., 1966: Problems of mandibular articulation fractures. Acta Stomatologica Belgica 63(3): 285-296

Park, S.H.; Gill, M.A.; Dopheide, J.A., 2003: Visual compatibility of risperidone solution and lithium citrate syrup. American Journal of Health-System Pharmacy 60(6): 612-613

Sandoval, M., A.; Stolpe, N., B.; Zagal, E., M.; Mardones, M., 2007: The effect of crop-pasture rotations on the C, N and S contents of soil aggregates and structural stability in a volcanic soil of South-Central Chile. The effects of six crop-pasture rotations were evaluated on properties of soil aggregates in a volcanic soil (Humic Haploxerand) of south-central Chile. Rotations that included intensive cropping without pastures, and crops with short-or long-ter...

Zheng, L.; Qu, W.; Zhang, J., 2017: A case report of subclinical hyperthyroidism progressed to severe Graves disease after ovarian stimulation intervention. Zhonghua Nei Ke Za Zhi 56(3): 208-209

Fryer, J.C.F., 1926: Lepidoptera in the Scilly Islands. Entomologist [London] 59(754): 82

Harris, T.N.; Harris, S.; Tulsky, E., 1959: Studies on the transfer of lymph node cells. XIII. Effect of x-irradiation on lymph node cells incubated in vitro with Shigella-trypsin filtrate. Journal of Immunology 82(1): 26-31

Johnson, C.; Ahsan, N.; Gonwa, T.; Halloran, P.; Stegall, M.; Hardy, M.; Metzger, R.; Shield, C.; Rocher, L.; Scandling, J.; Sorensen, J.; Mulloy, L.; Light, J.; Corwin, C.; Danovitch, G.; Wachs, M.; van Veldhuisen, P.; Salm, K.; Tolzman, D.; Fitzsimmons, W.E., 2001: Randomized trial of tacrolimus (Prograf) in combination with azathioprine or mycophenolate mofetil versus cyclosporine (Neoral) with mycophenolate mofetil after cadaveric kidney transplantation. Our clinical trial was designed to investigate the optimal combination of immunosuppressants for renal transplantation. A randomized three-arm, parallel group, open label, prospective study was performed at 15 North American centers to compare thr...