geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Injury-induced Janus kinase/protein kinase C-dependent phosphorylation of growth-associated protein 43 and signal transducer and activator of transcription 3 for neurite growth in dorsal root ganglion


, : Injury-induced Janus kinase/protein kinase C-dependent phosphorylation of growth-associated protein 43 and signal transducer and activator of transcription 3 for neurite growth in dorsal root ganglion. Journal of Neuroscience Research 85(2): 321-331

Elevation of corticosteroids and excessive glutamate release are the two major stress responses that occur sequentially during traumatic CNS injury. We have previously reported that sequential application of corticosterone and kainic acid (CORT + KA) mimicking the nerve injury condition results in synergistic enhancement of neurite outgrowth and expression of growth-associated protein 43 (GAP-43) in cultured dorsal root ganglion (DRG). GAP-43 is known to promote neurite extension when phosphorylated by protein kinase C (PKC). In addition PKC can phosphorylate the signal transducer and activator of transcription 3 (STAT3) at Ser727, which is phosphorylated primarily by Janus kinase (JAK) at Tyr705. In this study, we further examine the role of PKC in this stress-induced growth-promoting effect. In the cultured DRG neurons, the JAK inhibitor AG-490 and the PKC inhibitor Ro-318220 reduced the CORT + KA-enhanced neurite growth effect when applied prior to CORT and KA treatment, respectively. Both AG-490 and Ro-318220 diminished the CORT + KA-enhanced GAP-43 expression, phosphorylation, and axonal localization. Furthermore, CORT + KA treatment synergistically phosphorylated STAT3 at Ser727 but not at Tyr705. Similar phenomena were observed in an animal model of acute spinal cord injury (SCI), in which phosphorylation of GAP-43 and phospho-Ser727-STAT3 was elevated in the injured DRG 4 hr after the impact injury. Further treatment with the therapeutic glucocorticoid methylprednisolone enhanced the phosphorylation of GAP-43 in both the DRG and the spinal cord of SCI rats. These results suggest that elevated glucocorticoids and overexcitation following CNS injury contribute to nerve regeneration via induction of JAK/PKC-mediated GAP-43 and STAT3 activities. (c) 2006 Wiley-Liss, Inc.

US$19.90

PMID: 17131417

DOI: 10.1002/jnr.21119


Other references

Rokavec, M.; Schroth, W.; Amaral, S.M.C.; Fritz, P.; Antoniadou, L.; Glavac, D.; Simon, W.; Schwab, M.; Eichelbaum, M.; Brauch, H., 2008: A polymorphism in the TC21 promoter associates with an unfavorable tamoxifen treatment outcome in breast cancer. Tamoxifen therapy is a standard in the treatment of estrogen receptor (ER)-positive breast cancer; however, its efficacy varies widely among patients. In addition to interpatient differences in the tamoxifen-metabolizing capacity, there is growing...

Alha, A.L., 1954: Malignant chorionepithelioma, use of hysterosalpingography as a diagnostic aid; a case report. Annales Chirurgiae et Gynaecologiae Fenniae 43(2): 136-141

Mette, A., 1955: Symptomatology and theory of speech disorders in schizophrenia. Zhurnal Nevropatologii i Psikhiatrii Imeni S.S. Korsakova 55(11): 811-811

Lalli, A.F., 1969: Medullary sponge kidney disease. Radiology 92(1): 92-96

Johnson, B.; Sandberg, C., 1965: The profitability of milk production. A study of farm accounts from the book-keeping organization of Sveriges Lantbruks-forbund. The profitability in milk production in 1960-1965 is investigated using results from 100 large dairy farms in S. and Central Sweden which participated in a rather detailed book-keeping system. The yield per cow increased by 2 per cent. per year fr...

Cuevas Covarrubias, S.; Valdes Flores, M.; Jimenez Vaxca, A.L.; Kofman Alfaro, S.H., 2000: Point mutation in the exon 9 of the STS gene causing X-linked ichthyosis. European Journal of Human Genetics 8(Supplement 1): 111, June

Muñoz, L.M.; DeFeudis, F.V.; Fando, J.L., 1977: High-affinity binding of 3H-glycine and 14C-beta-alanine to synaptosomal-mitochondrial fractions of rat CNS regions; effects of strychnine. Neuropharmacology 16(7-8): 537-540

Pérez Ruiz, T.; Martínez-Lozano, C.; Tomás, V.; Carpena, J., 2008: Simultaneous determination of propranolol and pindolol by synchronous spectrofluorimetry. The simultaneous determination of propranolol and pindolol using synchronous fluorescence spectrometric techniques is described. The method involves measuring the natural fluorescence of these drugs in a 50% (v/v) ethanol-water medium using zero a...

Jiang, L.; Yamashita, Y.; Toyokuni, S., 2010: A novel method for efficient collection of normal mesothelial cells in vivo. Asbestos-induced mesothelioma is a challenging social problem in many countries, and oxidative stress via iron is closely associated with its carcinogenesis. Mesothelioma is thought to originate from the mesothelial cells that cover the somatic ca...

Zhou, H.; Feng, G.; Yao, K.; Yang, C.; Yi, J.; Zhou, S., 2013: Fiber-based tunable microcavity fluidic dye laser. We investigate a tunable fluidic dye laser formed by a microcavity filled with a dye solution. We achieve a wide 18 nm tunability of the laser wavelength by controlling the cavity length for the first time. The microcavity is made of a silica cap...