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Intraneuronal amyloid beta 42 enhanced by heating but counteracted by formic acid


, : Intraneuronal amyloid beta 42 enhanced by heating but counteracted by formic acid. Journal of Neuroscience Methods 159(1): 134-138

Amyloid beta-protein ending at 42 (A beta 42) is the major peptide deposited in Alzheimer's disease (AD) brain. In immunocytochemical studies, formic acid treatment is used to dramatically enhance A beta immunoreactivity. Recently, A beta 42 has been reported to accumulate in AD neurons. Since heating is known to enhance intracellular protein immunoreactivity, we used an autoclaving protocol to enhance intraneuronal A beta 42 immunoreactivity. Using this protocol, both anti-A beta 42 N-terminal and C-terminal antibodies, but not anti-A beta 40 C-terminal antibody, labeled AD neurons. Moreover, formic acid treatment counteracted such effects of autoclaving. Thus, intraneuronal A beta 42 accumulation may have been underestimated by conventional methods using formic acid only.

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Other references

Anyane Yeboa K.; Pallai M.; Taterka P., 1985: Inherited metabolic screening of newborns 7 years experience in a new york hospital usa. American Journal of Human Genetics 37(4 SUPPL): A210

Schulte, P.; Sanner, B., 2005: Falling asleep disorders after taking coffee?. Deutsche Medizinische Wochenschrift 130(20): 1276-1276

Bauer, Albert, 1967: New estimation of the Greenland ice sheet mass balance. The Greenland ice sheet mass balance is negative with an amount of water equivalent of -110 km3/yr; this represents an equivalent water sheet of +0.3 mm/yr uniformly spread over the seas of the globe. Recent data on the actual retreat of the Green...

Arrow, Gilbert J., 1948: A horned melolonthine beetle-from South America. CERATOLONTHA venezuelae (Melolonthinae) is descr. from Venezuela.

Gopal, K.; Ussher, J.R., 2017: Sugar-sweetened beverages and vascular function: food for thought. American Journal of Physiology. Heart and Circulatory Physiology 312(2): H285-H288

Clinkenbeard John P.; Churchill Ronald K., 2005: Guidelines for geologic investigations of naturally occurring asbestos in California. California has experienced a rising concern over potential public exposure to naturally occurring asbestos (NOA) in recent years. Consequently, geologists in California are increasingly called upon to evaluate the NOA potential of property prior t...

Bernath, A.M.; Cohen, M.H.; Ihde, D.C.; Fossieck, B.E.; Matthews, M.J.; Minna, J.D., 1976: Combination chemotherapy with methyl-CCNU, 5-fluorouracil, and vincristine in adenocarcinoma and large cell carcinoma of the lung. Cancer Treatment Reports 60(9): 1393-1394

Yang, T-Ching.; Hu, R-Mei.; Weng, S-Fen.; Tseng, Y-Hsiung., 2007: Identification of a hypothetical protein of plant pathogenic Xanthomonas campestris as a novel beta-galactosidase. Xc17L, a lactose-utilizing mutant of Xanthomonas campestris pv. campestris previously isolated by mutagenesis with nitrous acid, displays a level of beta-galactosidase 3.5-fold higher than that in the parental Xc17. In this study, the gene encodi...

Knowlton, G.F.; Taylor, E.J.; Hanson, W.J., 1948: Insect food of Uta stansburiana stansburiana in the Timpie area of Utah. Herpetologica Chicago, 4: 197-198

Singleton Michael J.; Criss Robert E.; Champion Duane E., 1998: Oxygen isotope imaging of three-dimensional flows in the Utah Mine, Comstock Lode mining district, Nevada. Abstracts with Programs - Geological Society of America 30(7): 77