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Purification and characterization of butyrate-induced protein phosphatase involved in apoptosis of Ehrlich ascites tumor cells


, : Purification and characterization of butyrate-induced protein phosphatase involved in apoptosis of Ehrlich ascites tumor cells. Biochimica et Biophysica Acta 1770(1): 39-47

Short chain fatty acids including butyrate exhibit wide variety of biological effects towards cell growth, morphology and gene expression. In this report, we study the mechanism by which butyrate (BuA) modulates the expression of protein phosphatase when treated to the cells. As a model system, we used Ehrlich Ascites Tumor (EAT) cells in which BuA-treatment induces expression of a protein phosphatase enzyme. Subsequently, BuA-induced protein phosphatase has been biochemically purified and characterized. Further, pretreatment of caspase-3 inhibitor abolished the activity of BuA-induced protein phosphatase indicating the involvement of caspase-3 in the activation of BuA-induced protein phosphatase. In addition, the relationship between BuA-induced protein phosphatase and apoptosis has been verified. Activation of endonuclease-II has been shown in BuA-treated EAT cells and that activity was completely inhibited by sodium orthovanadate, a tyrosine phosphatase inhibitor suggesting that endonuclease-II may serve as a possible down-stream target for BuA-induced protein phosphatase. Together, the data suggest that activation of protein phosphatase may be an early and essential step in BuA-mediated apoptotic signaling pathway in EAT cells.

US$19.90

PMID: 17029793

DOI: 10.1016/j.bbagen.2006.07.007


Other references

Mandelstam, S.L., 1967: Studies of shortwave solar radiation in the U.S.S.R. A history is presented of measurements of solar vacuum uv and soft x radiation made by rocket and satellite-borne instruments in the U.S.S.R. The origin and mechanism of the production of solar x rays are discussed.

Nakayama, D.K.; Davies, P.; Pitt, B.R.; Chough, V.H.; Simmons, R.L.; Billiar, T.R., 1992: Fetal pulmonary artery smooth muscle cells produce nitric oxide in response to lipopolysaccharide and cytokines. Surgical Forum 43(0): 623-626

Chessells M.; Hawker D.W.; Connell D.W., 1992: Influence of solubility in lipid on bioconcentration of hydrophobic compounds. Superhydrophobic compounds (log Kow > 6) do not bioconcentrate as much as predicted from the extrapolation of linear relationships between the logarithms of the bioconcentration factor (KB) and the octan-1-ol/water partition coefficient (Kow) d...

Mesterhazy, A., 2003: Control of Fusarium head blight of wheat fungicides. This chapter discusses the chemical control of Fusarium head blight. Some methods for evaluating fungicide efficacy are described. The results of different fungicide experiments are presented.

Fang, P.; Shiue, G.G.; Shimazu, T.; Greenberg, J.H.; Shiue, C-Yann., 2004: Synthesis and evaluation of N,N-dimethyl-2-(2-amino-5-[18F]fluorophenylthio)benzylamine (5-[18F]-ADAM) as a serotonin transporter imaging agent. The synthesis and evaluation of a new serotonin transporter (SERT) imaging agent, N,N-dimethyl-2-(2-amino-5-[18F]fluorophenylthio)benzylamine (5-[18F]-ADAM) is reported. Nucleophilic substitution of N,N-dimethyl-2-(2-nitro-5-bromophenylthio)benzyl...

Stark H.; Dienst M., 1989: Dynamics of lakeside reed belts at lake constance untersee western europe from 1984 to 1987. Since 1984 the German Association for Bird Protection has been investigating the development of lakeside reed borders at Lake Constance (Untersee). At 31 test areas, situated along the shore of the Untersee, an average progression of 15.5 cm could...

Maccormac, H., 1937: Molluscum Sebaceum. Proceedings of the Royal Society of Medicine 30(9): 1053-1054

Segarra, R.; Ojeda, N.; Zabala, A.; García, J.; Catalán, A.; Eguíluz, J.Ignacio.; Gutiérrez, M., 2012: Similarities in early course among men and women with a first episode of schizophrenia and schizophreniform disorder. The aims of this study were to analyze the presence of gender differences in the phenotypic expression of schizophrenia at the onset of illness and to explore whether these differences determine clinical and functional outcome 2 years after the in...

Iavisheva, T.M.; Khlynina, E.G.; Semeniak, O.Iu., 1999: Regulation of differentiation of epithelial cells in mouse intestines. Biulleten' Eksperimental'noi Biologii i Meditsiny 128(7): 39-41

Guzman Rolando A. de, 1966: Uranium mineralization in the Paracale mining district, Camarines Norte. Uranium mineralization in Larap and Dawahan areas in Paracale mining district, Camarines Norte, is associated with pyrometasomatic, replacement, and hydrothermal deposits related to diorite which was intruded during middle Miocene?. The principal...