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Quantification of serine enantiomers in rat brain microdialysate using Marfey's reagent and LGMS/MS


, : Quantification of serine enantiomers in rat brain microdialysate using Marfey's reagent and LGMS/MS. Journal of Chromatography B 846(1-2): 359-363

The ability to selectively measure serine enantiomer concentrations in rat brain microdialysate is essential during drug discovery to study the interaction of D-serine with the N-methyl-D-aspartate (NMDA) subtype of the glutamate receptor. NMDA receptor-stimulating agents, such as D-serine, have been shown to reduce the negative symptoms and cognitive dysfunction in individuals with schizophrenia when added to conventional or atypical antipsychotic drug regimens. In the work presented here, an LC/MS/MS assay was developed and validated to simultaneously measure D-serine and L-serine concentrations in rat brain microdialysate. Reverse phase chromatographic resolution of the enantiomers was obtained through derivatization with 1-fluoro-2,4-dinitrophenyl-5-L-alanine amide (Marfey's reagent). The assay was validated to determine concentrations over the range of 10-7500 ng/mL using electrospray ionization and multiple reaction monitoring (MRM). Both intra- and inter-day precision and accuracy were less than 16.5% (RE) and 7% (CV) for both analytes, respectively, and assay throughput was increased significantly relative to existing methodologies.

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Other references

Henry R.P., 1989: Membrane and cytoplasmic carbonic anhydrase in branchial and extra branchial tissues in aquatic and terrestrial crabs. American Zoologist 29(4): 153A

Oselladore, Guido, 1929: Possibility of transformation of endothelial cells into migratory histiocytes. In 8 rabbits the great omentum and a loop of the small intestine were aseptically exposed for 15 min.; then returned to the abdominal cavity and a suspension of India ink injected into the ear vein. The rabbits were bled to death 2 hrs. after the...

Foeldi M., 1977: New aspects in the therapy of lymph edema. Mayall, R C And Marlys H Witte (Ed ) Progress in Lymphology The Fourth And Fifth International Congresses Tuscon, Ariz , Usa, March 6-10, 1973 And Rio De Janeiro, Brazil, March 27-29, 1975 Xiv+389p Illus Plenum Press: New York, N Y , Usa; London, England Isbn 0-306-30989-0 299-302

Ambrose, K.R.; Gracely, R.H.; Glass, J.M., 2014: Fibromyalgia dyscognition: concepts and issues. Fibromyalgia is characterized by widespread pain and tenderness; however, comorbid cognitive difficulties are a common complaint among patients. Known as fibro fog or dyscognition, this symptom comprises difficulties with complex cognitive process...

Ruan, Y.W.; Fan, Y.; Du, Z.Q.; Xu, Z.C., 2002: Increased dendritic branching in ca1 pyramidal neurons after transient forebrain ischemia. CA1 pyramidal neurons in hippocampus die 2-3 days after transient cerebral ischemia. Electrophysiological studies have shown that the excitatory synaptic transmission in CA1 neurons was enhanced after ischemia. To investigate the morphological cha...

Vineberg, A., 1955: The importance of the nurse in the care of cardiac surgery. Canadian Nurse 51(9): 687-689

Konnerth Ionescu A., 1979: Outline of the hydrachnellae acari of romania. The genera and species of Hydrachnellae (Acari) from Romania are given, with their distribution and bibliography. [A total of 284 spp. are discussed.].

Delahaye-Sourdeix, M.; Anantharaman, D.; Timofeeva, M.N.; Gaborieau, Vérie.; Chabrier, Aélie.; Vallée, M.P.; Lagiou, P.; Holcátová, I.; Richiardi, L.; Kjaerheim, K.; Agudo, A.; Castellsagué, X.; Macfarlane, T.V.; Barzan, L.; Canova, C.; Thakker, N.S.; Conway, D.I.; Znaor, A.; Healy, C.M.; Ahrens, W.; Zaridze, D.; Szeszenia-Dabrowska, N.; Lissowska, J.; Fabianova, E.; Mates, I.Nicolae.; Bencko, V.; Foretova, L.; Janout, V.; Curado, M.Paula.; Koifman, S.; Menezes, A.; Wünsch-Filho, V.; Eluf, 2015: A rare truncating BRCA2 variant and genetic susceptibility to upper aerodigestive tract cancer. Deleterious BRCA2 genetic variants markedly increase risk of developing breast cancer. A rare truncating BRCA2 genetic variant, rs11571833 (K3326X), has been associated with a 2.5-fold risk of lung squamous cell carcinoma but only a modest 26% inc...

Reismanis, E.A., 1978: The problem of calculating fixed capital for land improvement and extra charges for amortization deductions in the Latvian SSR. The results of an investigation into accounting fixed means for land improvement in kolkhozy and sovkhozy and also in the control of land improvement systems are given. Difficulties encountered in determining the composition of inventory units for...

Yoder, K.E.; Espeseth, A.; Wang, X-hong.; Fang, Q.; Russo, M.Teresa.; Lloyd, R.Stephen.; Hazuda, D.; Sobol, R.W.; Fishel, R., 2011: The base excision repair pathway is required for efficient lentivirus integration. An siRNA screen has identified several proteins throughout the base excision repair (BER) pathway of oxidative DNA damage as important for efficient HIV infection. The proteins identified included early repair factors such as the base damage recog...