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Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural south Australia


, : Diagnosis of acute Q fever by PCR on sera during a recent outbreak in rural south Australia. Annals of the New York Academy of Sciences 1078: 566-569

Diagnosis of Q fever has largely been dependent upon serology, which may lead to delayed diagnosis as seroconversion can take weeks to develop. During a recent Q fever outbreak (27 patients) in rural South Australia, we compared the diagnostic rate of serology with two separate real-time PCRs, the 27kDa outer membrane protein and the insertion sequence. PCR was positive (on either or both PCR assays) in sera of 67% of the patients. Median time required for making serological diagnosis was 17 days, compared with 4 days by PCR. Q fever PCR is an effective tool in the diagnosis of acute Q fever infection.

US$19.90

PMID: 17114779

DOI: 10.1196/annals.1374.112


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