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Serum leptin and ghrelin levels in response to methylprednisolone injection in healthy dogs

, : Serum leptin and ghrelin levels in response to methylprednisolone injection in healthy dogs. Research in Veterinary Science 82(2): 187-194

The aim of this study was to investigate the effects of methylprednisolone treatment on serum leptin and ghrelin levels in healthy dogs (n = 40). After 14 h of fasting, the dogs were injected intramuscularly with saline (control group) or methylprednisolone (1, 5 or 10 mg/kg). Blood samples were collected prior to (baseline) and 2, 3, 4, 8, 12 and 24 h subsequent to the treatments. Serum leptin and ghrelin were measured by radioimmunoassay. The mean baseline serum leptin and ghrelin were 2.5 pl 0.1 ng/mL (n = 40) and 35.0 pl 2.1 pg/mL (n = 40), respectively. In the control dogs, serum leptin, but not ghrelin levels showed a significant fluctuation during the 24 h observation period. Serum leptin increased significantly (p < 0.05-0.01) between 2 and 12 h after 1 mg/kg of methylprednisolone. Serum leptin levels showed biphasic response to 5 mg/kg of methylprednisolone: its level decreased to 1.9 pl 0.1 ng/mL (p < 0.01) at 2 h and increased at 12 h (2.6 pl 0.1 ng/mL) (p < 0.01). In response to 10 mg/kg of methylprednisolone, serum leptin levels decreased significantly (p < 0.01) for 24 h. Serum ghrelin levels decreased to 19 pl 5 pg/mL at 2-3 h (p < 0.01) or increased to 87 pl 18 pg/mL at 3-8 h (p < 0.05-0.01) after 1 mg/kg of methylprednisolone or 10 mg/kg of methylprednisolone, respectively. Serum ghrelin levels did not change at any time point during 24 h observation period after 5 mg/kg of methylprednisolone. There was a significant (p < 0.001) inverse correlation (r = -0.635) between serum leptin and ghrelin levels. In conclusion, we found that methylprednisolone increases or decreases serum leptin and ghrelin levels depending upon its dose and there is a negative correlation between serum leptin and ghrelin levels after methylprednisolone administration.


PMID: 17014872

DOI: 10.1016/j.rvsc.2006.07.005

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