+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of lactic acid bacteria from vinegar flies based on phenotypic and genotypic characteristics

, : Identification of lactic acid bacteria from vinegar flies based on phenotypic and genotypic characteristics. American Journal of Enology and Viticulture 57(4): 519-525

Thirty lactic acid bacteria were isolated from the intestinal tract of Drosophila simulans Stuvervant collected from a winery in the Stellenbosch region, South Africa. The isolates were grouped according to morphological, biochemical, and physiological characteristics. Strains selected from each group were identified to species level by PCR with species-specific primers, PCR-based DGGE, and 16S rDNA sequencing. The majority of isolates belonged to the species Lactobacillus plantarum, but Lactobacillus paracasei, Lactobacillus sanfranciscensis, Leuconostoc mesenteroides subsp. mesenteroides, Lactococcus lactis subsp. lactis, Enterococcus faecalis and Pediococcus pentosaceus were also identified. The genotypic relatedness between strains of L. plantarum was determined by RAPD-PCR.


Other references

Rabanal, B.; D.A.riba, R.G.; Garzon, M.J.; Reguera, R.M.; Balana Fouce, R.; Negro, A., 1994: Determination of pentamidine in Leishmania infantum promastigotes by ion-paired liquid chromatography. Pentamidine is an aromatic diamidine, which have recently been shown to be non-competitive inhibitors of putrescine uptake in parasitic protozoa. In be to understand the involved in Pentamidine uptake by parasitic protozoa cells, a HPLC analytical...

Thomas, Ja, 1980: Quantitative analysis of the vocal repertoire of Weddell seals (Leptonychotes weddelli) in McMurdo Sound, Antarctica. Dissertation Abstracts International B Sciences and Engineering 40(11): 5130

Ravdin, I.S., 1946: Some experiences in Assam. Pharos of Alpha Omega Alpha-Honor Medical Society. Alpha Omega Alpha 10(1): 12-16; Zhou ZhongXuan; Barker, M.; Yang Xiu; Kong HongMei; Zhao JingZhu, 2003: Effect of intercropping distance on crop yield in a Paulownia-wheat agroforestry system in the Huang-Huai-Hai Plain, China. A case study in a Paulownia (Paulownia elongata)-wheat intercropping system in the Huang-Huai-Hai Plain, Henan, China, was conducted, and the relationship between tree belt distance (D), intercropping age (A) and annual relative wheat yield (Y) wa...

Anonymous, 1976: Proceedings of the ninth research-industry conference, American Forage and Grassland Council, Stauffers Inn, Louisville, Kentucky, February 9, 10, 11, 1976. Of the 23 papers presented at the conference, the following are noted.

Calman, W.T., 1928: Subterranean Crustacea. Journal of the Quekett Microscopical Club, 16(2): 1-8

Gagnon, K.Be.; Fyffe, R.Ew.; Adragna, N.C.; Lauf, P.K., 2007: Characterization of an extracellular epitope antibody to the neuronal K-Cl cotransporter, KCC2. 1. Ion gradients across the cell membrane are important for proper cellular communication and homeostasis. With the exception of erythrocytes, chloride (Cl), one of the most important free anions in animal cells, is not distributed at thermodynami...

D.D.os García, J.; Otero Gómez-Quintero, M., 1971: Etiopathogenic consideration on the choanal atresia. Acta Oto-Rino-Laringologica Ibero-Americana 22(4): 423-32 Passim

Pop, M.; Aktories, K.; Schmidt, G., 2004: Isotype-specific degradation of Rac activated by the cytotoxic necrotizing factor 1. The cytotoxic necrotizing factor 1 (CNF1) from Escherichia coli activates members of the Rho family by deamidation of glutamine 61/63. Because this amino acid is crucial for GTP hydrolysis, deamidation of glutamine 61/63 results in constitutively...

Dmitruk, I.; Shynkarenko, Y.; Dmytruk, A.; Aleksiuk, D.; Kadan, V.; Korenyuk, P.; Zubrilin, N.; Blonskiy, I., 2017: Efficiency estimates and practical aspects of an optical Kerr gate for time-resolved luminescence spectroscopy. We report experience of assembling an optical Kerr gate setup at the Femtosecond Laser Center for collective use at the Institute of Physics of the National Academy of Sciences of Ukraine. This offers an inexpensive solution to the problem of time...