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Effects of in vitro exposure to ozone and


, : Effects of in vitro exposure to ozone and. Aquaculture research 33(3): 165-175

In aquaculture, ozone is used as a disinfectant. In its production, extensive amounts of oxygen are formed resulting in hyperoxic conditions in culture units. Both ozone and hyperoxia have the potential to be toxic via pro-oxidant mechanisms and to activate antioxidant defence systems in cultured species. To eliminate systemic effects, blood of rainbow trout, Oncorhynchus mykiss (Walbaum), was exposed in vitro for 5 min to ozone/hyperoxia or hyperoxia, and changes in antioxidant defences and lipid peroxidation were measured after exposure. Ozone exposure caused severe damage in red blood cells (rbc) detected as increased lipid peroxidation and oxidized glutathione (GSSG) levels in both plasma and rbc. Oxygen exposure alone increased intracellular lipid peroxidation and GSSG levels 10 min after exposure and was not evident in the plasma at any time. Ozone, but not oxygen exposure, decreased reduced glutathione (GSH) levels in plasma, and the changes were negatively correlated with increased lipid peroxidation in rbc, indicating that extracellular GSH has a dynamic role in the protection of rbc from direct oxidation by ozone. Both ozone and hyperoxic conditions increased superoxide dismutase (SOD) activity in rbc 3 and 6 h after exposure. In contrast, catalase activity was only increased 10 min after oxygen exposure, suggesting other catalase activation mechanisms rather than enzyme induction. The recovery of lipid peroxidation and GSSG levels in rbc after hyperoxia, but not ozone exposure, indicated a capacity to defend against hyperoxia-produced oxidative damage, but an overwhelming of antioxidant defences by ozone in rainbow trout rbc in vitro.

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Other references

Brindley G.W., 1946: The basal Permian sands south of Wetherby. Notes the distribution of Permian basal sands in the Collingham area, Yorkshire, England, and describes exposures in several localities. Mechanical analyses of samples of the sand are reported, and it is noted that they are very uniform in grading...

Trouw Rudolph A.J., 1988: Structural analysis of the Elephant Island Group, South Shetland Islands. Serie Cientifica Instituto Antartico Chileno

Sanada, T.; Yoshida, Y.; Haniuda, T., 1981: Studies on the method of seed storage in apple breeding. III. Differences in seed storage capacity in varieties with different maturity dates. Results are reported of investigations involving 14 early, intermediate or late-maturing cultivars or wild species. Seed was subjected to short-term moist storage, submerged storage at 0 deg C, storage in the fruit and long-term dry storage. Seed...

Bouattour, A.; Darghouth, M.A.; Daoud, A., 2000: Distribution and ecology of ticks (Acari: Ixodidae) infesting livestock in Tunisia: an overview of eighth years field collections. Ticks (Ixodidae) play a significant role as vectors of pathogens of domestic animals in Tunisia. The major losses caused by ticks are related to transmission of protozoan parasites. These include agents of tropical theileriosis and babesiosis in r...

Luna Azoulay, B.; Béquignon, A.; Babin, E.; Moreau, S., 2009: Preliminary results of percutaneous tracheotomies. Percutaneous tracheotomy (PT) is an alternative to surgical tracheotomy (ST). We describe our procedure and discuss the current status of PT through a retrospective study of our first 30 cases. Thirty patients had a PT between October 2006 and Mar...

Thomas G.A., 1976: Faunal evidence of climatic change in the early Permian of the Carnarvon Basin, Western Australia. International Geological Congress, Abstracts--Congres Geologique Internationale, Resumes 25, Vol

Zeller, R.; Hertsch, B.; Wilkens, H.; Neurand, K.; Hartung, K., 1975: Specification of exposure directions for x-ray examination in veterinary medicine. Dtw. Deutsche Tierarztliche Wochenschrift 82(1): 22-24

Ibrahim, M.; Islam, M.R.; Banik, N.G., 1977: Effect of glucose tolerance test on blood glucose, non-esterified fatty acids and total serum proteins in diabetic patients. In 15 normal persons and 50 with adult-onset diabetes not dependent on insulin, 25 to 48 years old, blood was sampled fasting and 1 and 2 h after 50 g glucose by mouth. Mean concentrations in serum of fasting normal and diabetic persons were of gl...

Nowotny, H., 2011: What the European Research Council is looking for in applications. Bioessays 32(7): 545-547

Briere, J.; Rickards, S., 2007: Self-awareness, affect regulation, and relatedness: differential sequels of childhood versus adult victimization experiences. This study examined abuse and trauma exposure as it predicted identity problems, affect dysregulation, and relational disturbance in 620 individuals from the general population. Multivariate analyses indicated that maternal (but not paternal) emot...