+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Tick anticoagulant peptide: kinetic analysis of the recombinant inhibitor with blood coagulation factor Xa

, : Tick anticoagulant peptide: kinetic analysis of the recombinant inhibitor with blood coagulation factor Xa. Biochemistry 29(50): 11095-11100

Tick anticoagulant peptide (TAP) is a 60 amino acid protein which is a highly specific inhibitor of human blood coagulation factor Xa (fXa) isolated from the tick Ornithodoros moubata [Waxman, L., Smith, D. E., Arcuri, K. E., & Vlasuk, G. P. (1990) Science 248, 593-596]. Due to the limited quantities of native TAP, a recombinant version of TAP produced in Saccharomyces cerevisiae was used for a detailed kinetic analysis of the inhibition interaction with human fXa. rTAP was determined to be a reversible, slow, tight-binding inhibitor of fXa, displaying a competitive type of inhibition. The binding of rTAP to fXa is stoichiometric with a dissociation constant of (1.8 +/- 0.02) x 10(-10) M, a calculated association rate constant of (2.85 +/- 0.07) x 10(6) M-1 s-1, and a dissociation rate constant of (0.554 +/- 0.178) x 10(-3) s-1. Binding studies show that 35S-rTAP binds only to fXa and not to DFP-treated fXa or zymogen factor X, which suggests the active site of fXa is required for rTAP inhibition. That rTAP is a unique serine proteinase inhibitor is suggested both by its high specificity for its target enzyme, fXa, and also by its unique structure.


PMID: 2271697

Other references

Popoff, N., 1957: Anatomy of the nervous system in retrospect. The idea of the localization of the psychic functions inside the brain is old. As far back as the 5th century B. C, Alcmeon of Croton, a Greek, localized the perception of the sensations and of thought inside the brain. Other Greeks believed the d...

Matic, R.; Black, I.D., 1992: Integrated weed management systems for the control of sand fescue (Vulpia fasciculata) in barley in South Australia. Data are presented from field experiments conducted during 1989 and 1990 in South Australia on a sand loam soil, which assessed the effects of integrated methods for control of V. fasciculata in barley . V. fasciculata densities in direct-drilled...

Toyota, M.; Tagami, T.; Yasuda, N.; Ishikawa, Y.; Ohara, H., 1991: Detection of pulmonary tuberculosis. Special characteristics of patients detected while receiving treatment for other diseases. Tuberculosis (TB) detection is mainly classified as being either passive case-finding, at a hospital, or positive case-finding through a mass screening. Pulmonary TB patients detected while being examined for other complaints at a hospital are cla...

Quilter, J., 1985: Prehistory in peru: early ceremonial architecture in the andes. Science 230(4728): 931-932

Costantini, C.; Ale-Ali, A.; Helsten, T., 2011: Sleep aid prescribing practices during neoadjuvant or adjuvant chemotherapy for breast cancer. Sleep disruption is a common complaint in breast cancer patients receiving chemotherapy. We describe the sleep aid prescribing practices of oncologists treating women receiving adjuvant or neoadjuvant chemotherapy for breast cancer at a single ins...

Chernova, E.I., 1961: Biostratigraphy of Foraminifera of the Middle Carboniferous deposits of the Saratov right bank. Regionalnaya Stratigrafiya SSSR: 5: 261-286

Andonov, P.S., 1958: Virulence of tick-borne encephalitis virus in chicks. Voprosy Virusologii 3(5): 276-279

Perrino, F.W.; Mekosh, H.L., 1992: Incorporation of cytosine arabinoside monophosphate into DNA at internucleotide linkages by human DNA polymerase alpha. The incorporation of cytosine arabinoside monophosphate (araCMP) into DNA at internucleotide linkages by DNA polymerase alpha (DNA pol alpha) has been investigated by using oligonucleotide primed DNA templates. The products of reactions catalyzed...

Smith D.I.; Burvill P.W., 1990: Relationship between male pancreatitis morbidity and alcohol consumption in western australia australia 1971 84. Acute pancreatitis morbidity rates for Western Australian (Australia) men aged 20 to 39 years, and over 40 years, were related to per adult (.gtoreq. 15 years) alcohol consumption, using the Mann-Whitney U test. A significant result was obtained f...

Wetzel, H.; Fischer, P., 1971: Infection of a skin lesion in a dog caused by the blowfly Lucilia caesar (Linnaeus, 1758) (Diptera: Calliphoridae). Second-instar larvae of Lucilia caesar (L.) were found infesting a skin lesion in a dog. This is the first report of this fly causing myiasis in Germany. The third larval instar is described; it was not found to differ from that of L. illustris (M...