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CCAAT displacement protein regulates nuclear factor-kappa beta-mediated chemokine transcription in melanoma cells

, : CCAAT displacement protein regulates nuclear factor-kappa beta-mediated chemokine transcription in melanoma cells. Melanoma Research 17(2): 91-103

Members of the nuclear factor-kappa beta (NF-kappa B) family maintain cellular homeostasis by enhancing the transcription of genes involved in inflammation, immune response, cell proliferation, and apoptosis. Melanoma tumor cells often express inflammatory mediators through enhanced activation of NF-kappa B. The NF-kappa B activation appears to result from the enhancer formation including NF-kappa B and lysine acetyl transferases such as p300, CREB (cyclic AMP-responsive element binding protein)-binding protein (CBP), and/or p300/CBP associating factor (PCAF). We observed that proteins expressed by Hs294T metastatic melanoma cells are highly acetylated compared with normal melanocytes, and dominant-negative PCAF reduced the basal and tumor necrosis factor-alpha-stimulated transcriptional activity of NF-kappa B. The promoter activity of NF-kappa B-regulated chemokines was also reduced by the expression of dominant-negative PCAF. The promoters of these chemokines contain a CCAAT displacement protein (CDP)-binding site near the NF-kappa B element. compared with vector-transduced cells, in CDP-transduced Hs294T cells: over-expressed CDP bound efficiently to PCAF, (ii) tumor necrosis factor-alpha-stimulated chemokine expression and NF-kappa B-mediated transcription were reduced, and (iii) the binding of CBP to Rel A was reduced. These data suggest that CDP inhibits cytokine-induced NF-kappa B-regulated chemokine transcription. This study contributes to our understanding of the role of CDP in an enhanceosome of NF-kappa B-mediated chemokine transcription in human melanoma cells.


PMID: 17496784

DOI: 10.1097/CMR.0b013e3280a60888

Other references

Giardina, A.; Frosecchi, M.; Brat, A., 1963: On A Clinical Case Of Cerebral Infarct With An Ischemic-Like Electrocardiographic Finding. Physiopathologic Considerations. Rassegna di Neurologia Vegetativa 17: 73-83

Kuusio, H.; Lämsä, R.; Aalto, A-Mari.; Manderbacka, K.; Keskimäki, I.; Elovainio, M., 2015: Inflows of foreign-born physicians and their access to employment and work experiences in health care in Finland: qualitative and quantitative study. In many developed countries, including Finland, health care authorities customarily consider the international mobility of physicians as a means for addressing the shortage of general practitioners (GPs). This study i) examined, based on register...

Fitch, J.A.; Kumar, K.; Pinault, D.M., 1997: Ectoparasite control stick for domesticated animals. Official Gazette of the United States Patent & Trademark Office Patents 1198(1): 334-335, May 6

Oppenheim-Gluckman, H.; Marioni, G.; Virole, B.; Aeschbacher, M.T.; Canny-Verrier, F., 2003: Vecu des enfants dont l'un des parents est cerebrolese: Etude preliminaire. Objective: To explore the impact of the brain injury of a parent on his child's behavioural and emotional symptoms and personal experience. Patients and setting: Nineteen children from 6 to 12 years old at the time of the research having a br...

Frouz, Jan., 1993: Faunistic records from the Czech Republic - 8. Diptera: Chironomidae. Klapalekiana, 293-4: 168

Lindemann, W.; Handtmann, S.; Schulte, W.; Hüttemann, H., 1987: Signs of corrosion on silver posts in root canals (II). Deutsche Zahnarztliche Zeitschrift 42(7): 639-646

Bernhard, K.; Pedersen, W., 1963: Fatty acid synthesis in rat brain. Pure cerebrosides, sphingomyelines, lecithines and neutral fats were isolated from brains of young rats 6 and 72 hours after intracerebral injection of C14 acetate. The composition of their fatty acids was detected and determined the specific acti...

Authier F.; Desbuquois B.; Lederer F., 1990: Degradation of glucagon in liver endosomes in vivo and in vitro studies. Cell Biology International Reports 14(ABSTR SUPPL): 213

Uemura, H.; Tezuka, Y.; Hasegawa, C.; Kobayashi, H., 1994: Immunohistochemical investigation of neuropeptides in the central nervous system of the amphioxus, Branchiostoma belcheri. The immunohistochemical localization of nine different neuropeptides was studied in the central nervous system of the amphioxus, Branchiostoma belcheri. In the brain, perikarya immunoreactive for urotensin I and FMRFamide were localized in the vic...

Actis, S.; Czakon, Mł.; Gluza, J.; Riemann, T., 2008: Virtual hadronic and leptonic contributions to Bhabha scattering. Using dispersion relations, we derive the complete virtual QED contributions to Bhabha scattering due to vacuum polarization effects. We apply our result to hadronic corrections and to heavy lepton and top quark loop insertions. We give the first...