+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep

, : Measurement of ovine pregnancy-associated glycoprotein (PAG) during early pregnancy in Lacaune sheep. Reproduction in Domestic Animals 42(3): 257-262

This study describes ovine pregnancy-associated glycoprotein (ovPAG) concentrations in 20 Lacaune sheep during early pregnancy. Measurements were performed by using semi-purified ovPAG as standard, tracer and immunogens for antibody production in rabbits. Antisera R780 (against ovPAG(57+59kDa)) and R805 (against ovPAG5(58+61kDa)) were used respectively in RIA-780 and RIA-805. Blood samples were collected at days 0, 18, 20, 22 and 25 after artificial insemination. From day 18 after breeding onward, the mean ovPAG concentration was significantly higher (p < 0.001) in plasma samples from pregnant ewes (n = 17) than in non-pregnant ones (n = 3). The specific activity of the tracer was 11 760 Ci/mmol in RIA-780 and 14 900 Ci/mmol in RIA-805. The minimal detection limits for RIA-780 and RIA-805 were 0.2 ng/ml and 0.3 ng/ml, respectively. The intra-assay CV of samples with low (1.0 ng/ml), medium (2.5 ng/ml) and high (4.0 ng/ml) PAG concentrations were 3%, 6% and 9% for RIA-780 and 8%, 9% and 5% for RIA-805. The inter-assay CV in the same samples were 13%, 12% and 7% for RIA-780 and 13%, 11% and 5% for RIA-805. The recovery was higher than 95% in both assays. No cross-reaction was observed with members of aspartic proteinase family as well as with other tested proteins. In both RIA-780 and RIA-805, inhibition of the binding of the tracer by antisera was parallel between standard curve and serial dilutions of pregnant ewe samples. In conclusion, the two homologous RIA systems are suitable for early quantification of ovPAG concentrations in ewe plasma samples from day 18 after breeding.


PMID: 17506803

DOI: 10.1111/j.1439-0531.2006.00761.x

Other references

Ruiz, F.J., 1991: Relationships among length of productive life, milk yield, and profitability of United States, Canadian, and Mexican Holstein sires in Mexico. Length of productive life (LPL) of Mexican Holstein daughters of US, Canadian and Mexican sires was analysed, modelling their immediate probability of culling with the results incorporated into a lifetime net present value (PV$) sire model. Data w...

Silverstein, D.N.; Ingvar, M., 2015: A multi-pathway hypothesis for human visual fear signaling. A hypothesis is proposed for five visual fear signaling pathways in humans, based on an analysis of anatomical connectivity from primate studies and human functional connectvity and tractography from brain imaging studies. Earlier work has identif...

Bregulla, HL., 1984: Die gebanderte Ralle (Gallirallus philippensis sethsmithi). Gefiederte Welt, 1089: 246-248

Clarke, S.C.; McIntyre, M., 2001: Acid-fast bodies in faecal smears stained by the modified Ziehl-Neelsen technique. The modified Ziehl-Neelsen (ZN) stain has proved useful in the laboratory diagnosis of cryptosporidiosis and, more recently, for the laboratory diagnosis of Cyclospora. Many other organisms and artefacts are present in faeces and may be seen in fa...

Ballantyne, T., 1995: Airlines in Australasia. Trends in the commercial air transport sector in Australasia (Australia and New Zealand) suggest moves towards the creation of a single aviation market. The three major airlines in the region: Qantas, Air New Zealand and Ansett, may soon become tw...

Cosner, C., 2005: A dynamic model for the ideal-free distribution as a partial differential equation. One of the major approaches to understanding spatial effects in ecology and population dynamics is the use of analytic models. Some types of analytic models for spatial effects, such as dispersal models based on partial differential equations or i...

Yates, J.P.; McGill, S.M., 2011: The effect of vibration and posture on the progression of intervertebral disc herniation. Spine 36(5): 386-392

Ikemoto, H.; Murakami, M.; Terada, H.; Nakazawa, S.; Kamegaya, K.; Saito, T., 1963: Pulmonary Aspergilloma (The So-Called Fungus Ball)--Report Of 5 Cases. Naika. Internal Medicine 12: 515-525

Werner, J.K.; Barreto, P., 1981: Leishmaniasis in Colombia, a review. A review is made of 60 publications on leishmaniasis in Colombia reported between 1889 and the present. A serious source of difficulty in interpreting the literature is the frequent discussion of 'cases' (1536 of 1865, or 82%) that were...

Donaldson, I.M.L.; Redi, F., 2010: Redi's denial of spontaneous generation in insects. Journal of the Royal College of Physicians of Edinburgh 40(2): 185-186