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Relationship between neural, vascular, and BOLD signals in isoflurane-anesthetized rat somatosensory cortex


, : Relationship between neural, vascular, and BOLD signals in isoflurane-anesthetized rat somatosensory cortex. Cerebral Cortex 17(4): 942-950

Functional magnetic resonance imaging (fMRI) in anesthetized rodents has been commonly performed with alpha-chloralose, which can be used only for terminal experiments. To develop a survival fMRI protocol, an isoflurane (ISO) -anesthetized rat model was systematically evaluated by simultaneous measurements of field potential (FP) and cerebral blood flow (CBF) in the somatosensory cortex. A conventional forepaw stimulation paradigm with 0.3 ms pulse width, 1.2 mA current, and 3 Hz frequency induced 54% less evoked FP and 84% less CBF response under ISO than alpha-chloralose. To improve stimulation-induced responses under ISO, 10-pulse stimulations were performed with variations of width, current, and frequency. For widths of 0.1-5.0 ms and currents of 0.4-2.0 mA, evoked FP and CBF increased similarly and reached a plateau. The evoked FP increased monotonically for intervals from 50 to 500 ms, but the CBF peaked at an interval of 83 ms (approximately 12 Hz frequency). These data suggest that different anesthetics profoundly affect FP and CBF responses in different ways, which requires optimizing stimulation parameters for each anesthetic. With the refined stimulation parameters, fMRI consistently detected a well-localized activation focus at the primary somatosensory cortex in ISO-anesthetized rats. Thus, the ISO-anesthetized rat model can be used for cerebrovascular activation studies, allowing repeated noninvasive survival experiments.

US$19.90

PMID: 16731882

DOI: 10.1093/cercor/bhl005


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Baran E.Ya; Krimkevich E.I.; Zagrab'yan R.O., 1988: Antithrombotic drugs in the multimodality treatment of acute rejection kidney allograft. During three first months after kidney transplantation the parameters of three conjugated enzymatic systems of plasma: hemocoagulatory, fibrinolytic and kallikrein-kinin were studied in 101 patients for the evaluation of their role in the impairme...

Dike, MC., 1991: Courtship behaviour in two species of Atherigona Rondani (Dipt., Muscidae). Indian Journal of Entomology. June; 532: 257-261

Nahoum Grappe, Y., 1995: Portrait of a war Former Yugoslavia since 1991. Medecine et Armees 23(5): 469-480

Hall, C.B.; Burdine, H.W., 1957: Evaluation of factors determining celery quality. Two short notes are presented. In the first work on the chemical constituents of celery is outlined, and in the second the effects of harvesting at 4 stages of maturity on yield, pithiness, petiole thickness, rib length and cracking are reported.

Collins D.A.; Pacumbaba R.P., 1984: Races of phytophthora megasperma f sp glycinea phytophthora root rot of soybeans in northern alabama usa. Phytopathology 74(7): 852-853