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Phagocytosis employed as a serological test in brucellosis


, : Phagocytosis employed as a serological test in brucellosis. Ada Path El Microbiol Scand 18(1): 103-110

This is a description of a new cytophagic test, in which heated serum from the patient, is used, rather than whole blood as in Huddleson's test. The leucocytes are obtained from normal citrated blood. The patient's serum is heated at 56[degree]C for 30 min. The amt. used for the test is 0.02 cc to which is added 0.1 cc bacterial suspension (1 cm. Gates). The tube is shaken and incubated in a water bath for 30 min. at 37[degree]C after which 0.1 cc non-phagocytic blood is added and reincubated for 30 min. at 37[degree]C. Slides are then made, air dried and stained with aqueous methylene blue. 0.2%, for 10 mins. Advantages of the technique: the patient's blood does not have to be used immediately after collection; use of heated serum eliminates some nonspecific reactions; it reveals an inhibition of phagocytosis which sometimes occurs when the opsonin content of the blood is high. The test is more sensitive than the agglutination and complement-fixation tests, and reveals the presence of immune opsonins at an early stage of the disease.

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Other references

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Wheeler, E.J., 1947: Effective potato vine spray and dusts check disease and make harvesting easier. Leaf and vine killing checks late blight epidemics and tuber rot promotes drying of the soil and facilitates harvesting. The same spraying or dusting equipment may be used as was used during the growing season. The chemicals are not named as exptl...

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Geambasu, A.; Krukoff, T.L., 2008: Adrenomedullin acts in the lateral parabrachial nucleus to increase arterial blood pressure through mechanisms mediated by glutamate and nitric oxide. Adrenomedullin (ADM) acts in a site-specific manner within autonomic centers of the brain to modulate mean arterial pressure (MAP). To determine the role of ADM in the pontine autonomic center, the lateral parabrachial nucleus (LPBN), we used uret...

Mussell, M.J.; Paulev, P.E.; Miyamoto, Y.; Nakazono, Y.; Sugawara, T., 1990: A constant flux of carbon dioxide injected into the airways mimics metabolic carbon dioxide in exercise. This paper reports the expired minute-ventilation (VE) responses of 5 subjects to three step levels in a) work rate on a bicycle ergometer (30, 50, and 70 W), b) inhaled constant fraction (CF) of CO2 (3, 5, and 7%), and c) inhaled constant flux (C...

Tzolov, V.P.; Fontaine, M.; Godbout, N.; Lacroix, S., 1995: Nonlinear self-phase-modulation effects: a vectorial first-order perturbation approach. A full-vectorial integral expression is derived to compute the effective mode area of any Kerr-type nonlinearoptical waveguide working in a self-phase-modulation regime. In order to highlight the correction brought by the vectorial approach in a s...

Fujinami, K.; Kondo, I.; Miura, T.; Harada, M.; Uemura, H.; Hosaka, M.; Kawasaki, C., 1998: Prognostic significance of clinical stage including extent of disease (EOD) in prostate cancer. We analyzed the prognostic significance of clinical stage using extent of disease (EOD) grading system in 288 patients with prostate cancer between 1970 and 1994. The cause-specific survival rate for EOD4 was significantly lower (p < 0.01) than...

Li, J.; Huang, S.; Armstrong, E.A.; Fowler, J.F.; Harari, P.M., 2005: Angiogenesis and radiation response modulation after vascular endothelial growth factor receptor-2 (VEGFR2) blockade. The formation of new blood vessels (angiogenesis) represents a critical factor in the malignant growth of solid tumors and metastases. Vascular endothelial cell growth factor (VEGF) and its receptor VEGFR2 represent central molecular targets for a...

Coquil, J.F.; Brunelle, G.; Guédon, J., 1985: Occurrence of the methylisobutylxanthine-stimulated cyclic GMP binding protein in various rat tissues. A new type of cGMP binding protein, the activity of which is characteristically stimulated by methylisobutylxanthine, has been previously discovered in rat lung and platelets (Hamet, P. and Coquil, J.F. (1978) J. Cyclic Nucleotide Res. 4, 281-290)...