geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

The expression and promoter methylation of SLIT2 gene in human primary hepatocellular carcinomas


, : The expression and promoter methylation of SLIT2 gene in human primary hepatocellular carcinomas. Fudan Xuebao (Yixueban) 34(2): 202-206

Purpose To study the SLIT2 expression and mechanism of its silencing in hepatocellular carcinoma (HCC). Methods Genomic DNA and total RNA were extracted from 3 normal liver tissue samples, 32 HCC samples and 8 liver cancer cell lines, respectively. The expression of SLIT2 was evaluated by semi-quantitative RT-PCR. The methylation specific PCR (MSP) and bisulfite DNA sequencing were performed to examine the methylation status of SLIT2 gene promoter. Results Promoter methylation of SLIT2 gene was found in 27 of 32 liver tumor tissues (84.3%) and 18 of 32 non-cancerous liver tissues ( 56.3%) from 32 HCC patients, and 6 of 8 liver cancer cell lines ( 75%), whereas SLIT2 methylation was not detected in normal liver tissues from healthy controls. There was no correlation between promoter hypermethylaion and pathological data for primary HCC patients, including tumor size, lymph metastasis, and clinical stage. However, the frequency of methylation in elder patients (>= 60 years old) was significantly higher than that in younger patients (< 60 years old) (P < 0.05). Interestingly, the expression of SLIT2 could be induced by the demethylating agent 5-aza-2-deoxycytidine in HCC cell lines with the promoter hypermethylation of SLIT2 gene. Conclusions Promoter hypermethylation of SLIT2 gene may occur as an early event in the development of HCC. Methylation is possibly a major mechanism for inactivation of SLIT2 gene in HCC.

US$29.90


Other references

Chokroverty, S.; Deutsch, A.; Guha, C.; Gonzalez, A.; Kwan, P.; Burger, R.; Goldberg, J., 1995: Thoracic spinal nerve and root conduction: a magnetic stimulation study. We describe a technique of magnetic coil (MC) stimulation of the thoracic spinal nerves and roots in 12 normal subjects and a patient with diabetes mellitus. We kept the MC flat against the vertebral column in the midline over T-7, T-8, and T-9 sp...

Giuliano, K.K., 2003: Expanding the use of empiricism in nursing: can we bridge the gap between knowledge and clinical practice?. The philosophy of Aristotle and its impact on the process of empirical scientific inquiry has been substantial. The influence of the clarity and orderliness of his thinking, when applied to the acquisition of knowledge in nursing, can not be overs...

Xiaoxia Wang; Tao Wang; Antonio Castro; Rocío Pedreira; Xinxiang Lu; Qinghui Xiao, 2011: Triassic granitoids of the Qinling orogen, central China Genetic relationship of enclaves and rapakivi-textured rocks. Granitoids varying in composition from biotite amphibole granodiorite to monzogranite with rapakivi textures have recently been recognized in the Qinling orogen (central China). The granitoids are magnesian, calc-alkalic and metaluminous (A/CNK=.7...

Roesch, B.; Corcoran, M.; Haffey, M.; Stevenson, A.; Wang, P.; Purkayastha, J.; Martin, P.; Ermer, J., 2014: Pharmacokinetics of coadministration of guanfacine extended release and methylphenidate extended release. ?<sub>2</sub>-Adrenoceptor agonists are used adjunctively to psychostimulants in treating attention-deficit/hyperactivity disorder (ADHD) when psychostimulants alone do not sufficiently reduce symptoms. However, data on the pharmacokin...

Anonymous, 1932: Methods of sowing sunflower and maize for silage and their treatment. A distance between drills and plants of 50 cm. and 10 cm. respectively was found to be advisable for sunflower, while for maize the distances should be 60 and 20 cm., in order to allow for mechanization in treatment.

Rahimi,E.; Momtaz,H.; Bonyadian,M., 2010: PCR detection of Campylobacter sp from turkey carcasses during processing plant in Iran. Turkey carcasses were sampled in one commercial poultry processing plant of Isfahan (Iran) to evaluate the contamination of carcasses to <i>Campylobacter</i> spp. along the processing plant by PCR method. A total of 348 samples were co...

Barber, P.J.; Elliott, J., 1996: Study of calcium homeostasis in feline hyperthyroidism. Thirty cats with untreated hyperthyroidism were blood sampled and their calcium homeostatic mechanisms and renal function assessed. The results were compared with those obtained from 38 age-matched control cats. The hyperthyroid group of cats were...

Fisher, H.A., 1993: Management of penile carcinoma: the case for selective application of inguinal lymph node dissection in stages T1-T4. Seminars in Urology 11(2): 74-79

Turrentine, J.E.; Dharamsi, J.W.; Miedler, J.D.; Pandya, A.G., 2011: Acute generalized exanthematous pustulosis (AGEP) caused by telavancin. Journal of the American Academy of Dermatology 65(3): E100-E101

Faye, R.M.; Sawadogo, S.; Mora Camino, F., 2002: Fuzzy logic applied to long term management of water resource systems. Since the origins of history, irrigation of agricultural lands has been reported to be an activity of great concern for many human societies. At the beginning, natural irrigation systems such as the Nile River flowing through the sands of Egypt ha...