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Purification studies on the infectious canine hepatitis virus. I. Viral particles in tissue culture fluid revealed by one purification procedure


, : Purification studies on the infectious canine hepatitis virus. I. Viral particles in tissue culture fluid revealed by one purification procedure. Japanese Jour Vet Res 8(2): 208-212

Infectious canine hepatitis virus, strain "Matsuda", from dog kidney tissue culture has been purified by methanol precipitation, extraction with organic solvents, and differential centrifugation. The ICH virus particle in the resulting purified virus concentrates has been identified by electron microscopy in agar pseudo-replica specimens as a uniform-sized particle 85 mu (unshadow), 96 mu (Pd-shadow) in diameter. These particles were found in parallel with viral infectivity. Some of the particles contain a central dense core surrounded by less dense material enclosed in a membrane when unshadowed and this central core when shadowed appears to be protuberant.

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Other references

Dorjee, S.; Heuer, C.; Jackson, R.; West, D.M.; Collins-Emerson, J.M.; Midwinter, A.C.; Ridler, A.L., 2009: Are white-spot lesions in kidneys in sheep associated with leptospirosis?. To determine the association between white-spot lesions in kidneys and serological and cultural prevalence of leptospirosis in sheep, and to evaluate the diagnostic value of these lesions in individual sheep and lines of sheep at slaughter as indi...

Tang, Z.; Maroto Valer, M.M.rcedes; Zhang, Y.; Kuchta, M.E.; Bair, S.A.; Andresen, J.M., 2002: Simultaneous coal gasification and CO2 sequestration using a fluidized bed of activated serpentine. Abstracts of Papers American Chemical Society 223(1-2): FUEL 17

Castelli, F.; Conti, B.; Maccarrone, D.E.io; Conte, U.; Puglisi, G., 1998: Comparative study of 'in vitro' release of anti-inflammatory drugs from polylactide-co-glycoside microspheres. A differential scanning calorimetry study has been carried out on the effect exerted by three anti-inflammatory drugs, Biphenylacetic Acid, Naproxen, and Ketoprofen, released from polylactide-co-glycolide (50:50 w:w) microspheres (loaded with two...

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Piller L.W., 1971: Electronic instrumentation theory of cardiac technology. Electronic Instrumentation Theory Of Cardiac Technology 224

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Anonymous, 2011: Comparison of within hive sampling and seasonal activity ofNosema ceranaein honey bee colonies. Nosema ceranaeis a microsporidian parasite of the European honey bee,Apis mellifera, that is found worldwide and in multipleApisspp.; however, little is known about the effects ofN. ceranaeonA. mellifera. Previous studies using spore counts sugges...

Jordà, J.; Rojas, H.Cueto.; Carnicer, M.; Wahl, A.; Ferrer, P.; Albiol, J., 2014: Quantitative Metabolomics and Instationary 13C-Metabolic Flux Analysis Reveals Impact of Recombinant Protein Production on Trehalose and Energy Metabolism in Pichia pastoris. Pichia pastoris has been recognized as an effective host for recombinant protein production. In this work, we combine metabolomics and instationary 13C metabolic flux analysis (INST 13C-MFA) using GC-MS and LC-MS/MS to evaluate the potential impac...

Kazemi R., 1975: Importance of animal proteins in various stages of broiler growth. Poultry Science: 1736

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