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Simultaneous estimation of plasma volume, red cell volume and thiocyanate space in unanesthetized normal and splenectomized rats


, : Simultaneous estimation of plasma volume, red cell volume and thiocyanate space in unanesthetized normal and splenectomized rats. American Journal of Physiology 185(3): 441-445

A method for simultaneous estimation of plasma volume, red cell volume, and thiocyanate space in unanesthetized rats utilizing a polyethylene catheter in the common carotid artery with T-1824, P32-tagged red cells, and NaSCN was perfected and determinations were performed on 77 male albino rats. Determinations of plasma volume using T-1824 or I131-serum albumin in the same animal gave essentially identical results. Total blood volume was calculated as the sum of P32-red cell volume and T-1824-plasma volume and was found to be 5.75 ml/100 g body weight in normal rats and 5.61 ml/100 g in splenectomized rats. There was a wide variation in the F cell values of normal rats but little in splenectomized rats. This difference was highly significant and was attributed to the presence of the spleen. The average thiocyanate space was found to be 33.0 ml and 35.8 ml/100 g in normal and splenectomized rats, respectively.

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PMID: 13339969


Other references

Pap G.S., 1970: Congenital defect of scalp and skull in 3 generations of 1 family case report. Plastic & Reconstructive Surgery 46(2): 194-196

Eira, C.; Miquel, J.; Vingada, Jé.; Torres, J., 2006: Spermiogenesis and spermatozoon ultrastructure of the cestode Mosgovoyia ctenoides (Cyclophyllidea: Anoplocephalidae), an intestinal parasite of Oryctolagus cuniculus (Lagomorpha: Leporidae). Ultrastructural characters in spermiogenesis and spermatozoa are considered important tools to elucidate the phylogenetic relationships within the Platyhelminthes. In the Anoplocephalidae, ultrastructural data refer to the spermatozoon of 14 speci...

Papadopoulos P.V.; Finlay J.B.; Bourne R.B.; Andreae P., 1984: Tibial strains in the osteoarthritic knee in vitro. Orthopaedic Transactions 8(2): 343-344

Robinson, J.B.D.; Devonshire, C.R., 1954: Weedicide observational trial. PCP, with or without added 2, 4-D substantially reduced weed growth under coffee, especially when applied both before and after weed emergence, but caused some chemical tainting of the coffee.

Oyetunji, T.A.; Haider, A.H.; Obirieze, A.C.; Fisher, M.; Cornwell, E.E.; Qureshi, F.G.; Abdullah, F.; Nwomeh, B.C., 2014: Epidemiology of 577 pediatric firearm fatalities: a 2-year review of the National Trauma Data Bank. The purpose of this study was to delineate the epidemiology of pediatric firearm injuries, including ethno-demographic patterns with impact on years of potential life lost (YPLL). A 2-year review of the National Trauma Data Bank (2007 to 2008) was...

Glette, J.; Solberg, C.O.; Lehmann, V., 1982: Factors influencing human polymorphonuclear leukocyte chemiluminescence. Divalent cations (Mg2+, Ca2+) regenerated chemiluminescence (CL) of zymosan-stimulated human polymorphonuclear leukocytes (PMNs) abolished by 0.1 mM EGTA. Phenol red, erythrocytes and free haemoglobin reduced CL. To obtain maximal CL response, the...

Navrátil, Tás.; Sebková, S.; Kopanica, M., 2004: Voltammetry of lead cations on a new type of silver composite electrode in the presence of other cations. A new type of silver composite electrode was examined, prepared from silver, graphite powder, and methacrylate resin. The effects of the presence of various cations (cadmium, copper, bismuth, thallium), anions (chlorides), surface-active substance...

Shetty, M.; Narendra, A.Moti.Venkata.Raja.; Adiraju, K.Prasad.; Modugu, N.Rao., 2016: Study of Aplastic Anaemia with Cyclosporine in Resource Poor Setting. Aplastic Anaemia (AA) is a syndrome characterized by peripheral pancytopenia with hypo-cellular marrow. Acquired idiopathic AA is the most common variety, probably of an autoimmune aetiology. Bone Marrow Transplantation (BMT) is the treatment of c...

Duerre J.A.; Chakrabarty S., 1975: Distribution of methylated basic amino acid residues in histones from the various organs of the rat. Federation Proceedings 34(3): 610

Prazdny, S., 1980: A computational study of a period of infant object-concept development. Piaget has distinguished a number of distinct stages in the development of the concept of an enduring external object during infancy. I present a theory of a class of behaviours at one of these stages embodied in a working computer program. The be...