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Adaptation of the Rimini-Schryver reaction for the measurement of allantoin in urine to the autoanalyzer: allantoin and taurine excretion following neutron irradiation

, : Adaptation of the Rimini-Schryver reaction for the measurement of allantoin in urine to the autoanalyzer: allantoin and taurine excretion following neutron irradiation. Analytical Biochemistry 27(2): 333-342

The automatic method is based on alkaline hydro lysis of allantoin to allantoic acid which is subse quently hydrolysed with acid to urea and glyoxylic acid. The phenylhydrazone of glyoxylic acid is oxidized in strong acid with ferricyanide and absorb ance is measured at 520 m micro. Preliminary results show the effect of irradiation on excretion of allantoin and taurine by rats; average values before irradiation in micro moles per 24 h are 127 and 17 for female and 109 and 45 for male rats.


PMID: 5767191

DOI: 10.1016/0003-2697(69)90043-8

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