geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons


, : A rapid high-throughput method for the detection and quantification of RNA editing based on high-resolution melting of amplicons. Nucleic Acids Research 35(17): E114-E114

We describe a rapid, high-throughput method to scan for new RNA editing sites. This method is adapted from high-resolution melting (HRM) analysis of amplicons, a technique used in clinical research to detect mutations in genomes. The assay was validated by the discovery of six new editing sites in different chloroplast transcripts of Arabidopsis thaliana. A screen of a collection of mutants uncovered a mutant defective for editing of one of the newly discovered sites. We successfully adapted the technique to quantify editing of partially edited sites in different individuals or different tissues. This new method will be easily applicable to RNA from any organism and should greatly accelerate the study of the role of RNA editing in physiological processes as diverse as plant development or human health.

US$19.90

PMID: 17726051

DOI: 10.1093/nar/gkm640


Other references

Ruiz Sanchez Francisco Javier, 2005: Environmental legislation and protection of the paleontologic heritage in Valencia (Spain). Revista Espanola de Paleontologia Special issue 10: 119-124

Torriente, I.; Valdes Sosa, M.; Ramirez, D.; Bobes, M.A., 1999: Visual evoked potentials related to motion-onset are modulated by attention. The effects of attention on visual evoked potentials triggered by motion-onset were examined in four experiments. A set of randomly oriented bars was used as stimuli. The first experiment showed that responses to motion-onset following pattern-ons...

Funder, J.W.; Funder, J.W.; Mantero, F.; Ulick, S., 1994: A tale of three cities. Steroids 59(7): 400-403

Gjonnes, B.S., 1963: In vitro cultures of Picea abies: some trials with seedlings.. Discusses techniques and media for culture of seeds, excised embryos, etc., especially the avoidance of infection by microflora and of pigmentation (attributed to a non-enzymatic Cu-activated process); pigmentation could be retarded by tyrosine, b...

Ben yamovskiy V.N. ); Volchegurskiy L.F., 1979: The paleontological characteristics of the zonal subdivisions of the Upper Cretaeous in the eastern Peri-Caspian region. Pages 98-133, 156 1979

Cleland, J.B., 1945: Black Cockatoos with red crests. Emu Melbourne, 45: 99

Shalek, P.A.; Vinogradov, B.M.; Pozdniakov, A.V.; Stukov, L.A.; Garmashov, I.A., 2008: Methods and results of stereotactic radiosurgery for cavernous angiomas. Stereotactic radiosurgery was performed in 42 patients (1997-2007). They presented with 47 cavernous angiomas and 44 of them underwent surgery. Single focal dosage varied 3-9 Gy while total one--18-54Gy. After treatment and 2-year followup, neurol...

López Jiménez, P.; Vargas Nevado, Á.; Herrera Ceballos, E., 2016: Bullous Pemphigoid on the Areola of Breast. Actas Dermo-Sifiliograficas: -

Lee, R.E., 2003: Variety: 'Conlee' syn Autumn Amethyst. Application no. 2001/093. Conlee (syn. Autumn Amethyst) is a new Rhododendron sp. cultivar derived from the cross, seed parent cv. Karens x pollen parent R. oldhamii cv. Fourth of July. It is selected for its flowering time and flower colour. It is submitted for the regist...

Denis Theophile Constant, 1902: Occurrences of platinum in Canada. Annual Report - Geological Survey of Canada Pages 99-110