+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Amplification of moderately repetitive DNA sequences during chick cartilage differentiation

, : Amplification of moderately repetitive DNA sequences during chick cartilage differentiation. Proceedings of the National Academy of Sciences of the United States of America 73(10): 3428-3432

A 5-bromo-2'-[3H]deoxyuridine (BrdUrd) probe was isolated to analyze DNAs obtained from various chick tissues and cell types. [3H]BrdUrd-substituted DNA, prepared from limb bud cultures, was sheared and freed from palindromic DNA. Nonradioactive DNA was prepared from embryonic liver, undifferentiated limb bud mesenchyme, sternal cartilage, differentiated limb bud cultures, and BrdUrd-blocked cultures, and was sheared. These DNAs were used in 100-fold excess to drive the reassociation of the [3H]-BrdUrd-DNA probe. Purified mature cartilage DNAs of embryonic sternae or differentiated limb bud cultures drove the reassociation of the probe approximately two times faster than did DNA from liver, undifferentiated limb bud, or BrdUrd-blocked cells. These data indicate that cartilage DNA contains a greater number of sequences complementary to the BrdUrd probe than do DNAs of noncartilage or undifferentiated precartilage cells. Calculations determined an average substitution of 10% of thymidine residues by BrdUrd in purified probe, whereas CsCl density gradients of unsheared probe revealed radioactive peaks of greater than 20% substitution. The BrdUrd appears to be clustered in the genome.


PMID: 1068455

DOI: 10.2307/66612

Other references

Briggs, B.A.; McCulloch, S.M.; Caton, L.A., 1994: In vitro propagation of rhododendron. Techniques for the successful micropropagation of rhododendrons are considered from initiation into culture through multiplication to rooting ex vitro. Shoot tip culture has proved the most reliable propagation method. The impact of rhododendron m...

Huffines W.D., 1967: Splenomegaly analgesic ingestion and renal disease rat human acetophenetidin cent depress. New England Journal Of Medicine4: 749-751

Nilsson, F., 1954: News from the National Horticultural Experimental Center. The beginning of horticultural research in Sweden and its subsequent development, which now includes experiments on the improvement of fruit trees, berries, vegetables and ornamental plants by breeding and various cultural and other methods, carri...

Lüchtrath, H.; Seitz, H.M., 1990: Embolized Schistosoma ova in the lung. Der Pathologe 11(5): 304-306

Kaplan B.S.; D.C.adarevian J P., 1977: Classification of hemolytic uremic syndrome. Pediatric Research 11(4): 553

Wright, H.E.; Dorrell, I.B., 2014: Morning versus evening rectal temperature response in rabbits. Journal of Bacteriology 54(1): 91-91

Biernacki, M.P.; Tarnowski, A.; Lengsfeld, K.; Lewkowicz, Rł.; Kowalczuk, K.; Dereń, M., 2013: +Gz load and executive functions. Pilots are constantly exposed to a number of environmental factors, which include +Gz load. Physiological changes evoked by +Gz stimuli have already been well investigated in aviation medicine. However, the influence of +Gz stimulation on executiv...

Senoner, Z.; Saradi, J.; Banhegyi, D.; Bognar, C., 1996: Rhodococcus equi caused by lung abscess and empyema in a HIV-1 infected patient. Acta Microbiologica et Immunologica Hungarica 43(2-3): 178

Aldor, T.A.; Lander, H., 1963: Experience With The Baker-Hughes Multiple Retrieving Gastro-Intestinal Biopsy Tube: Incorporating Some Minor Modifications. Medical Journal of Australia 2: 1037-1040

Bharathidasan, T.; Mandalam, A.; Balasubramanian, M.; Dhandapani, P.; Sathiyanarayanan, S.; Mayavan, S., 2015: Zinc Oxide-Containing Porous Boron-Carbon-Nitrogen Sheets from Glycine-Nitrate Combustion: Synthesis, Self-Cleaning, and Sunlight-Driven Photocatalytic Activity. We developed a single-step thermal method that enables successful inclusion of ZnO components in the porous boron-carbon-nitrogen (BCN) framework to form a new class of functional hybrid. ZnO-containing BCN hybrids were prepared by treating a mixt...