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Discovery of a novel styrene monooxygenase originating from the metagenome


, : Discovery of a novel styrene monooxygenase originating from the metagenome. Applied and Environmental Microbiology 73(18): 5832-5839

Oxygenases form an interesting class of biocatalysts, as they typically perform oxygenations with exquisite chemo-, regio-, and/or enantioselectivity. It has been observed that, once heterologously expressed in Escherichia coli, some oxygenases are able to form the blue pigment indigo. We have exploited this characteristic to screen a metagenomic library derived from loam soil and identified a novel oxygenase. This oxygenase shows 50% sequence identity with styrene monooxygenases from pseudomonads (StyA). Only a limited number of homologs can be found in the genome sequence database, indicating that this biocatalyst is a member of a relatively small family of bacterial monooxygenases. The newly identified monooxygenase catalyzes the epoxidation of styrene and styrene derivatives and forms the corresponding (S)-epoxides with excellent enantiomeric excess [e.g., (S)-styrene oxide is formed with >99% enantiomeric excess, ee] and therefore is named styrene monooxgenase subunit A (SmoA). SmoA shows high enantioselectivity towards aromatic sulfides [e.g., (R)-ethyl phenyl sulfoxide is formed with 92% ee]. This excellent enantioselectivity in combination with the moderate sequence identity forms a clear indication that SmoA from a metagenomic origin represents a new enzyme within the small family of styrene monooxygenases.

US$19.90

PMID: 17644649

DOI: 10.1128/AEM.02708-06


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Casalone, C.; Bozzetta, E.; Caramelli, M.; Guardo, G. di; Eleni, C.; Santagada, G.F.; Loria, G., 2001: Lesion profile in ovine scrapie. In the present study, central nervous system (CNS) lesion profiles were examined and compared in a number of scrapie-affected sheep from five different disease outbreaks located in as many Italian regions, with the main aim of gaining some prelimi...

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Elliott, R.C.; Reed, W.D.C., 1968: Studies of high-concentrate diets for cattle. 1. Growth and food intake of steers fed on diets containing different levels of low-quality roughage. In feeding trials for 112 and 102 days diets for bullocks 7 to 11 or 19 to 23 months old had 5, 20, 35 or 50% roughage. The diets with 20 or 35% gave greatest gain and intakes of feed and digestible energy. Roughage content gave wide difference in...

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