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Distinct nuclear genes for yeast mitochondrial and cytoplasmic methionyl-tRNA synthetases


, : Distinct nuclear genes for yeast mitochondrial and cytoplasmic methionyl-tRNA synthetases. Biochemical and Biophysical Research Communications 85(4): 1392-1399

Total methionine-tRNA synthetases from wild type Saccharomycescerevisiae can be fractionated on hydroxylapatite into two peaks: Peak I is the mitochondrial, peak II the cytoplasmic isoenzyme. The specificity towards various tRNAs and the antigenic determinants are not identical. A mutant strain, known for its altered cytoplasmic enzyme, contains a mitochondrial species with the same properties as the wild type mitochondrial enzyme, as well as a cytoplasmic isoenzyme with a KM for methionine about 300 times higher than the corresponding wild type enzyme. Another strain, obtained by back-crossing the mutant with a wild type strain, retains the enzyme pattern found in the mutant. The results are in favor of two distinct nuclear genes for yeast mitochondrial and cytoplasmic methionyl-tRNA synthetases.

US$19.90

PMID: 84671

DOI: 10.1016/0006-291x(78)91158-0


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