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Effect of mannitol on growth of embryogenic callus-derived somatic embryos of carnation (Dianthus caryophyllus L.)


, : Effect of mannitol on growth of embryogenic callus-derived somatic embryos of carnation (Dianthus caryophyllus L.). Journal of Science and Technology of Agriculture and Natural Resources 11(1(A)): 179-186

In vitro regeneration of four carnation cultivars, namely Nelson, Impulse, Sagres and Spitit, through somatic embryogenesis was studied. MS culture medium was supplemented with 30 g sucrose, 2 mg 2,4-D and 0.2 mg BA/litre and used for embryogenic callus formation.

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