geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression in periplasmic space of Shewanella oneidensis


, : Expression in periplasmic space of Shewanella oneidensis. Protein Expression and Purification 56(1): 80-84

A Sheivanella expression system has been used for an overproduction of c-type multiheme proteins. The proteins were exported to the periplasmic space for the maturation. Since the periplasmic expression system is attractive, especially for protease-sensitive proteins, an expression vector containing a signal peptide was constructed for expressions in the periplasmic space of Shelvanella oneidensis. To evaluate the system, two eukaryotic proteins which originally do not have signal sequences and are difficult to express in Escherichia coli, were selected. The first is human cytochrome c. Properties of the recombinant cytochrome c were identical to those previously reported, indicating the protein is intact. The other was potato calcium-dependent protein kinase. The protein was expressed in periplasmic space. These results indicated that the system is generally applicable for any protein expression including c-type cytochromes, pro tease- sensitive proteins and those with multi-disulfide bonds because of transportation to the periplasmic space. (c) 2007 Etsevier Inc. All rights reserved.

US$19.90

PMID: 17689099

DOI: 10.1016/j.pep.2007.06.005


Other references

Anonymous, 1987: Simultaneous replication of hbv and hdv in chronic hbsag carriers. Journal of Hepatology 5

Rao, G.M., 1977: Efficiency and effectiveness of gamma rays and EMS in rice. Grains of the rice varieties TKM6, Norin 27, IARI10017 and AR4-87, and of var. spontanea were irradiated with gamma rays at 25 krad or treated with ethyl methanesulphate (EMS) at 0.6% for 6 h. In the M2 there was no difference between the two trea...

Barry, W.; Davies, H.E., 1968: A simple double-pulse isolation unit for use with a students' nerve stimulator. Journal of Physiology 195(2): 1p-1p

Albuquerque, A.C. de; Almeida, J.C. de; Cunha, F.M. da; Pereira, K.C.A.; Paz, R.C. da; Lima, E.A.L.A., 2003: Determination of infective doses of entomopathogenic fungi for bioassays with sugarcane termites. Laboratory studies were conducted to determine the infective rates of Metarhizium anisopliae var. anisopliae and Metarhizium flavoviride var. flavoviride on Nasutitermes sp. The inoculation in the insects was made by direct contact, on dishes with...

Chimuka, L.; Michel, M.; Cukrowska, E.; Buszewski, B., 2009: Influence of temperature on mass transfer in an incomplete trapping supported liquid membrane extraction of triazole fungicides. The influence of temperature in a supported liquid membrane (SLM) extraction of triazole fungicides was investigated. The mass transfer parameters such as diffusion coefficient, flux and apparent viscosity were determined at temperatures ranging f...

Suarez, Sán.; Doctorovich, F.; Harvey, M.Angel.; Baggio, R., 2013: Tetraaqua(4,4'-dimethyl-2,2'-bipyridine-κ2N,N')nickel(II) sulfate monohydrate: a simple molecule with an extremely complex hydrogen-bonding scheme. The title ionic compound, [Ni(C12H12N2)(H2O)4]SO4·H2O, is composed of an Ni(II) cation coordinated by a chelating 4,4'-dimethyl-2,2'-bipyridine ligand via its two N atoms [mean Ni-N = 2.056 (2) Å] and by four aqua ligands [mean Ni-O =...

Mand, P.; Miller, K.M., 2009: Upside-down vitreolysis. We describe a technique for performing laser vitreolysis in patients lying upside down on a gurney.

Perrett, D., 1976: Simplified low pressure high resolution nucleotide analyzer. A simple nucleotide analyzer was constructed from commercially available components. A 20 cm .times. 0.4 cm I.D. [inside diameter] column of pellicular anion exchanger operating at approximately 20 psi was found to perform identically with publish...

Kazubski, SL.; Dryl, S., 1974: Cztery Migdzynarodowy Kongres Protozoologiczny w Clemont-Ferrand. (2-9.9 1973 r.). Kosmos Warsz Ser A: 231: 86-90

Abdurakhmanov, B.A.; Kaĭnova, A.S., 1979: DNA and RNA of blood plasma in the evaluation of the activity of rheumatoid arthritis. Terapevticheskii Arkhiv 51(7): 29-33