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Expression in periplasmic space of Shewanella oneidensis

, : Expression in periplasmic space of Shewanella oneidensis. Protein Expression and Purification 56(1): 80-84

A Sheivanella expression system has been used for an overproduction of c-type multiheme proteins. The proteins were exported to the periplasmic space for the maturation. Since the periplasmic expression system is attractive, especially for protease-sensitive proteins, an expression vector containing a signal peptide was constructed for expressions in the periplasmic space of Shelvanella oneidensis. To evaluate the system, two eukaryotic proteins which originally do not have signal sequences and are difficult to express in Escherichia coli, were selected. The first is human cytochrome c. Properties of the recombinant cytochrome c were identical to those previously reported, indicating the protein is intact. The other was potato calcium-dependent protein kinase. The protein was expressed in periplasmic space. These results indicated that the system is generally applicable for any protein expression including c-type cytochromes, pro tease- sensitive proteins and those with multi-disulfide bonds because of transportation to the periplasmic space. (c) 2007 Etsevier Inc. All rights reserved.


PMID: 17689099

DOI: 10.1016/j.pep.2007.06.005

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Anonymous, 1987: Simultaneous replication of hbv and hdv in chronic hbsag carriers. Journal of Hepatology 5

Rao, G.M., 1977: Efficiency and effectiveness of gamma rays and EMS in rice. Grains of the rice varieties TKM6, Norin 27, IARI10017 and AR4-87, and of var. spontanea were irradiated with gamma rays at 25 krad or treated with ethyl methanesulphate (EMS) at 0.6% for 6 h. In the M2 there was no difference between the two trea...

Barry, W.; Davies, H.E., 1968: A simple double-pulse isolation unit for use with a students' nerve stimulator. Journal of Physiology 195(2): 1p-1p

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Perrett, D., 1976: Simplified low pressure high resolution nucleotide analyzer. A simple nucleotide analyzer was constructed from commercially available components. A 20 cm .times. 0.4 cm I.D. [inside diameter] column of pellicular anion exchanger operating at approximately 20 psi was found to perform identically with publish...

Kazubski, SL.; Dryl, S., 1974: Cztery Migdzynarodowy Kongres Protozoologiczny w Clemont-Ferrand. (2-9.9 1973 r.). Kosmos Warsz Ser A: 231: 86-90

Abdurakhmanov, B.A.; Kaĭnova, A.S., 1979: DNA and RNA of blood plasma in the evaluation of the activity of rheumatoid arthritis. Terapevticheskii Arkhiv 51(7): 29-33