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Expression of c-kit in human osteosarcoma and its relevance as a prognostic marker


, : Expression of c-kit in human osteosarcoma and its relevance as a prognostic marker. Journal of Clinical Pathology 60(7): 804-807

Aims: To examine the prognostic relevance of c-kit expression in human osteosarcomas and to evaluate the mutation status in exon 9 and exon 11 of the c-kit gene.Methods: c-kit expression was examined in 100 human osteosarcomas by immunohistochemistry using paraffin embedded tumour tissues, and capillary sequencing of genomic DNA was performed to search for mutations in exons 9 and 11 of the c-kit gene.Results: 20 osteosarcomas showed c-kit expression ranging from 5% to 90% (mean 5.9%; SD 16.74%). Furthermore, DNA sequences of exon 9 and exon 11 of the c-kit gene were not altered in these tumours. Overall and disease free survival analysis did not reveal any differences between patients with osteosarcoma with c-kit expression and those with c-kit negative tumours.Conclusions: C-kit expression is not a prognostic marker in patients with osteosarcoma. The protein expression is not linked to mutations in exon 9 or exon 11 of the c-kit gene. Therefore, these exons may not function as targets for treatment modalities based on the suppression of c-kit tyrosine kinase activity.

US$19.90

PMID: 17018686

DOI: 10.1136/jcp.2005.032839


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Carr, I.M.; Robinson, J.I.; Dimitriou, R.; Markham, A.F.; Morgan, A.W.; Bonthron, D.T., 2010: Inferring relative proportions of DNA variants from sequencing electropherograms. Determination of the relative copy number of single-nucleotide sequence variants (SNVs) within a DNA sample is a frequent experimental goal. Various methods can be applied to this problem, although hybridization-based approaches tend to suffer fro...

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