+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Expression of c-kit in human osteosarcoma and its relevance as a prognostic marker

, : Expression of c-kit in human osteosarcoma and its relevance as a prognostic marker. Journal of Clinical Pathology 60(7): 804-807

Aims: To examine the prognostic relevance of c-kit expression in human osteosarcomas and to evaluate the mutation status in exon 9 and exon 11 of the c-kit gene.Methods: c-kit expression was examined in 100 human osteosarcomas by immunohistochemistry using paraffin embedded tumour tissues, and capillary sequencing of genomic DNA was performed to search for mutations in exons 9 and 11 of the c-kit gene.Results: 20 osteosarcomas showed c-kit expression ranging from 5% to 90% (mean 5.9%; SD 16.74%). Furthermore, DNA sequences of exon 9 and exon 11 of the c-kit gene were not altered in these tumours. Overall and disease free survival analysis did not reveal any differences between patients with osteosarcoma with c-kit expression and those with c-kit negative tumours.Conclusions: C-kit expression is not a prognostic marker in patients with osteosarcoma. The protein expression is not linked to mutations in exon 9 or exon 11 of the c-kit gene. Therefore, these exons may not function as targets for treatment modalities based on the suppression of c-kit tyrosine kinase activity.


PMID: 17018686

DOI: 10.1136/jcp.2005.032839

Other references

Carr, I.M.; Robinson, J.I.; Dimitriou, R.; Markham, A.F.; Morgan, A.W.; Bonthron, D.T., 2010: Inferring relative proportions of DNA variants from sequencing electropherograms. Determination of the relative copy number of single-nucleotide sequence variants (SNVs) within a DNA sample is a frequent experimental goal. Various methods can be applied to this problem, although hybridization-based approaches tend to suffer fro...

Lin W.; Fang Z.; Jin Z Y., 1991: Modification of ethylene vinyl acetate copolymer and its effect on permeability of some steroids. The appropriate condition of mechanical blending for modification of ethylene-vinyl copolymer (EVA) with methylvinyl siloxane was reported. The melting heats of EVA and modified EVA (mEVA) were measured and the results indicated that the crystalli...

Lindahl, E.R., 2008: Molecular dynamics simulations. Molecular simulation is a very powerful toolbox in modern molecular modeling, and enables us to follow and understand structure and dynamics with extreme detail--literally on scales where motion of individual atoms can be tracked. This chapter foc...

Reid John A., 1918: Silver deposition and enrichment at Cobalt, Ont. (discussion). Economic Geology Pages 385-392

Takagi, M.; Amorim, C.R.N.; Ferreira, H.; Yano, T., 1997: Virulence related characteristics of Escherichia coli from sows with mastitis-metritis-agalactia (MMA) syndrome. Virulence-related characteristics of 30 E. coli strains isolated from sows with mastitis-metritis-agalactia (MMA) syndrome were examined. 18 E. coli strains agglutinated guineapig erythrocytes in the absence of D-mannose. 11 strains had type 1 fim...

Goldstein, P.A.; Storey-Johnson, C.; Beck, S., 2014: Facilitating the initiation of the physician's professional identity: Cornell's urban semester program. Calling for major reform in medical education, the Carnegie Institute report 'Educating Physicians' espoused the importance of assisting student trainees in forming their professional identities. Here, we consider the question: At what e...

Pina-Oviedo, S.; Torres-Cabala, C.A.; Miranda, R.N.; Tetzlaff, M.T.; Singh, S.; Rapini, R.P.; Prieto, V.G.; Aung, P.P., 2016: Concomitant Cutaneous Langerhans Cell Hystiocytosis and Leukemia Cutis. NlmCategory="UNASSIGNED">Leukemia cutis develops in <4% of all acute leukemias. Concurrent acute myeloid leukemia (AML) and Langerhans cell histiocytosis (LCH) is rare, with most cases involving lymph nodes or spleen, and no cutane...

Paepke, H.-Joachim.; Heym, W.-Dieter., 2002: Guppys in the Lower Lausitz Guppys in der Niederlausitz. DATZ. Marz; 553: 14-15

Lindsay Willard L.; Vlek Paul L.G.; Chien Sen H., 1989: Phosphate minerals.; 2. Pages 1089-1130 1989

Sulladmath, V.V.; Kulkarni, B.G.P.; Hegde, R.K., 1975: Some studies on chemical control of pineapple disease of sugarcane. The cut ends of sugar cane setts were dipped in benomyl 0.05% or the standard Agallol 0.5% for different durations of up to 5 min, and the extent of penetration of the fungicides was assessed. Benomyl penetrated up to 8 mm even when setts were dip...