+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Fermentation of biomass-generated synthesis gas: effects of nitric oxide

, : Fermentation of biomass-generated synthesis gas: effects of nitric oxide. Biotechnology and Bioengineering 97(5): 1080-1086

The production of renewable fuels, such as ethanol, has been steadily increasing owing to the need for a reduced dependency on fossil fuels. It was demonstrated previously that biomass-generated synthesis gas (biomass-syngas) can be converted to ethanol and acetic acid using a microbial catalyst. The biomass-syngas (primarily CO, CO(2), H(2), and N(2)) was generated in a fluidized-bed gasifier and used as a substrate for Clostridium carboxidivorans P7(T). Results showed that the cells stopped consuming H(2) when exposed to biomass-syngas, thus indicating that there was an inhibition of the hydrogenase enzyme due to some biomass-syngas contaminant. It was hypothesized that nitric oxide (NO) detected in the biomass-syngas could be the possible cause of this inhibition. The specific activity of hydrogenase was monitored with time under varying concentrations of H(2) and NO. Results indicated that NO (at gas concentrations above 40 ppm) was a non-competitive inhibitor of hydrogenase activity, although the loss of hydrogenase activity was reversible. In addition, NO also affected the cell growth and increased the amount of ethanol produced. A kinetic model of hydrogenase activity with inhibition by NO was demonstrated with results suggesting there are multiple binding sites of NO on the hydrogenase enzyme. Since other syngas-fermenting organisms utilize the same metabolic pathways, this study estimates that NO < 40 ppm can be tolerated by cells in a syngas-fermentation system without compromising the hydrogenase activity, cell growth, and product distribution.


PMID: 17171719

DOI: 10.1002/bit.21305

Other references

Metzger, J.; Hirsch, J.F.; Duttine, G.; Poirier, M.; Feldman, L.; Haddad, K.; Allal, M., 1980: Metrizamide radiculography with simplified dynamic test. The development of metrizamide, a recent hydrosoluble contrast medium, has opened up the possibility of dynamic lumbosacral radicolugraphy, but in the opinion of the authors only the upright position is adequate to accentuate or reveal new patholo...

Fischesser, B., 1980: Forests in the countryside. Bulletin technique d'information: (347 348) 251-258

Belles, A.; Alary, C.; Criquet, J.; Ivanovsky, A.; Billon, G., 2016: Assessing the transport of PAH in the surficial sediment layer by passive sampler approach. NlmCategory="UNASSIGNED">A new method based on passive samplers has been developed to assess the diffusive flux of fluorene, fluoranthene and pyrene in the sediment bed and across the sediment-water interface. The dissolved compound c...

L.T.; Pierard G.E.; Pierard Franchimont C.; D.L.B.assinne M.; Lapiere C.M., 1984: Investigations and treatment of cutaneous t cell lymphomas. Sixteen cases of cutaneous T cell lymphoma consisting of 11 cases with mycosis fungoides and 5 cases with high-grade malignant lymphomas are reported. A standardized clinical evaluation is proposed for patients with low-grade malignant lymphomas,...

Pepine, C.J.; Schang, S.J.; Bemiller, C.R., 1974: Effects of perhexiline on coronary hemodynamic and myocardial metabolic responses to tachycardia. Circulation 49(5): 887-893

Pierce J.A.; Eisen A.Z.; Dhingra H.K., 1969: Pathogenesis of emphysema in anti trypsin deficiency. Clinical Research 17(2): 477

de Castro, E.Aparecida.Barbosa.; de Camargo Junior, K.Rochel., 2008: Ethnography of health care after hospital discharge. This paper presents an analysis of how Clifford Geertz' anthropological approach contributes to studies and investigations on health care. Geertz' approach relies basically on a semiotic conception of culture adopting thick description a...

Bassett, D.J.; Reichenbaugh, S.S., 1992: Tricarboxylic acid cycle activity in perfused rat lungs after O2 exposure. O2-induced impairment of mitochondrial energy generation was examined in intact lungs isolated from rats after 18-30 h exposure to either air or 100% O2 in vivo. Mitochondrial metabolic rates were determined by separate measurements of 14CO2 produ...

Norrby-Teglund, A.; Newton, D.; Kotb, M.; Holm, S.E.; Norgren, M., 1994: Superantigenic properties of the group A streptococcal exotoxin SpeF (MF). Streptococcal pyrogenic exotoxin F (SpeF), previously referred to as mitogenic factor, is a newly described potent mitogen produced by group A streptococci. To investigate whether this protein belongs to the family of microbial superantigens, we a...

Bari, W.; Song, Y-Jae.; Yoon, S.Sun., 2011: Suppressed induction of proinflammatory cytokines by a unique metabolite produced by Vibrio cholerae O1 El Tor biotype in cultured host cells. Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-B...