+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of active cell in potassium transporting epithelium

, : Identification of active cell in potassium transporting epithelium. Journal of Experimental Biology 77: 1-13

Epithelial ion transport regulates the environment of cells and modulates the environment inside them, making possible the evolution of eukaryotic organisms. Analysis of the transport process requires that the route taken by ions as they are actively transported across epithelia be known. The route can be determined by locating ion pumps electrically and by measuring transport pool sizes kinetically provided that the epithelial structure is simple enough to enable the results to be interpreted in cellular terms. The route by which potassium is actively transported across the lepidopteran midgut is demonstrated here by a combination of electrical and kinetic analyses. Two sorts of cells, goblet and columnar cells, are distinguished by their electrical properties. The goblet cell is identified as the site of active potassium transport, the pump being localized in its apical membrane. The goblet cells are electrically coupled with columnar cells only when midguts that have been isolated from diet-reared larvae are short circuited. A corollary to this result is that the size of the pool of potassium which is involved in transport should be small when the goblet cells are not coupled with columnar cells and should become large when they are coupled. This corollary has been confirmed by direct measurement of the transport pools using isotope tracer kinetic analysis. This is the first time that the cell type responsible for active ion transport in a polymorphic tissue has been identified directly by physiological means.


PMID: 752058

Other references

Schneider, J.; Matsuoka, M.; Takeuchi, M.; Zhang, J.; Horiuchi, Y.; Anpo, M.; Bahnemann, D.W., 2014: Understanding TiO2 photocatalysis: mechanisms and materials. Chemical Reviews 114(19): 9919-9986

Fallis, A.M., 1952: Stages of Leucocytozoon simondi in black flies and ducks. Transc. troMed. Hyg: 378

Anonymous, 1971: Abstracts of papers given at the Hundred and Sixty-fourth Meeting of the Society held on 18th and 19th December 1970 at University College, London. CLARKE, B. C. The evolution of proteins: 340. CLEGG, J. B. The evolution of horse haemoglobins: 340. DICKINSON, A. G.

Wolters, G.; Stapert, S.; Brands, I.; Van Heugten, C., 2011: Coping styles in relation to cognitive rehabilitation and quality of life after brain injury. This study investigated the changes in coping styles of patients with acquired brain injury who underwent cognitive rehabilitation, and the effects of these changes on their quality of life. Participants were 110 patients in the chronic phase post...

Kiprov D.D.; Morand P., 1982: Lymphocyte sub population and mitogenic response studies in homo sexuals with acquired immuno deficiency syndrome. Immunobiology 163(2-4): 377

Bath, D., 1976: Production grouping means regular cow movement. Dairy herd management: 13 (10) 36-39

Cockcroft, P.D., 1995: Focal cystic endometrial hyperplasia in a bitch. Rapid abdominal enlargement and weight gain occurred in a four-year-old Rhodesian ridgeback bitch in late oestrus. The bitch was nulliparous. Severe cardiovascular dysfunction followed and an exploratory laparotomy was performed. A large, single,...

Popovich, M.J.; Lockrem, J.D.; Zivot, J.B., 1996: Nasal bridle revisited: an improvement in the technique to prevent unintentional removal of small-bore nasoenteric feeding tubes. Objective: To demonstrate the efficacy and safety of an updated version of the nasal "bridle," which is used to prevent the accidental removal of small-bore nasoenteric feeding tubes. Design: A descriptive study. Setting: Surgical intens...

Schiavetti, E., 1955: Antibiotic and roentgen therapy of osteomyelitis of mandible in an infant: course and results. Minerva Pediatrica 7(21-22): 655-657

Bocage, J.V.Barboza Du, 1865: Noticia acerea dos Arvicolas de Portugal. This memoir was presented to the Royal Academy of Sciences of Lisbon in 1861, and will be published in the ' Memoires' of this Academy. Meanwhile, it has been separately printed as a part of 'Memorias zoologicas,' apparently fo...