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Identification of the dual specificity and the functional domains of the cardiac-specific protein kinase TNNI3K


, : Identification of the dual specificity and the functional domains of the cardiac-specific protein kinase TNNI3K. General Physiology and Biophysics 26(2): 104-109

Molecular cloning of cardiac troponin I-interacting kinase (TNNI3K), a novel cardiac-specific protein kinase containing seven N-terminal ankyrin (ANK) repeats followed by a protein kinase domain and a C-terminal Ser-rich domain, has previously been reported. In the present study, we show that the C-terminal functional region of TNNI3K negatively regulates the kinase activity, and the N-terminal ANK domain is necessary for autophosphorylation. An in vitro kinase assay shows that TNNI3K exhibits dual-specific kinase activity and forms dimers or oligomers that may be necessary for its activation.

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PMID: 17660584


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