+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Linear derivatives of Saccharomyces cerevisiae chromosome III can be maintained in the absence of autonomously replicating sequence elements

, : Linear derivatives of Saccharomyces cerevisiae chromosome III can be maintained in the absence of autonomously replicating sequence elements. Molecular and Cellular Biology 27(13): 4652-4663

Replication origins in Saccharomyces cerevisiae are spaced at intervals of approximately 40 kb. However, both measurements of replication fork rate and studies of hypomorphic alleles of genes encoding replication initiation proteins suggest the question of whether replication origins are more closely spaced than should be required. We approached this question by systematically deleting replicators from chromosome III. The first significant increase in loss rate detected for the 315-kb full-length chromosome occurred only after all five efficient chromosomal replicators in the left two-thirds of the chromosome (ARS305, ARS306, ARS307, ARS309, and ARS310) had been deleted. The removal of the inefficient replicator ARS308 from this originless region caused little or no additional increase in loss rate. Chromosome fragmentations that removed the normally inactive replicators on the left end of the chromosome or the replicators distal to ARS310 on the right arm showed that both groups of replicators contribute significantly to the maintenance of the originless chromosome. Surprisingly, a 142-kb derivative of chromosome III, lacking all sequences that function as autonomously replicating sequence elements in plasmids, replicated and segregated properly 97% of the time. Both the replication initiation protein ORC and telomeres or a linear topology were required for the maintenance of chromosome fragments lacking replicators.


PMID: 17452442

DOI: 10.1128/MCB.01246-06

Other references

Karp, C.L.; Wysocka, M.; Wahl, L.M.; Ahearn, J.M.; Cuomo, P.J.; Sherry, B.; Trinchieri, G.; Griffin, D.E., 1996: Mechanism of suppression of cell-mediated immunity by measles virus. The mechanisms underlying the profound suppression of cell-mediated immunity (CMI) accompanying measles are unclear. Interleukin-12 (IL-12), derived principally from monocytes and macrophages, is critical for the generation of CMI. Measles virus (...

Derenyi, E.Y.zdani, R.R.erson, R., 1982: Monitoring the growth of crops using digital Landsat MSS data. Proceedings of the International Symposium on Remote Sensing of Environment First Thematic Conference: "Remote sensing of arid and semi arid lands" 19-25 January 1982 Cairo Egypt: 753

Bepler, G., 2003: Lung cancer: provoking new concepts, generating novel ideas, and rekindling enthusiasm. Cancer Control 10(4): 275-276

Hardjono, A., 1986: Properties of soils affecting cocoa growth on the Bunisari estate. In a trial from 1975 until 1984 on the Bunisari estate, West Java, cocoa seedlings (clone DR 1) budded with clone DR 2 were grown on 6 soil types (latosols or regosols). Physical and chemical analyses are tabulated. All types were considered suita...

Davids; Woods; Penttilä; Batchelder; Bingham; Blumenthal; Brown; Busse; Conticchio; Davinson; Henderson; Irvine; Seweryniak; Toth; Walters; Zimmerman, 1996: Proton decay of an intruder state in 185Bi. Physical Review Letters 76(4): 592-595

Shah, J., 1990: Taxonomic studies in the genus swertia l. gentianaceae monograph part i. The account deals with the Taxonomic studies of 77 species of Swertia L. (Gentianaceae) of the World.

Mckim, Jm, 1981: A direct measure of the uptake efficiency of xenobiotic chemicals across the respiratory epithelium of brook trout (Salvelinus frontinalis). Canadian Technical Report of Fisheries and Aquatic Sciences: 108-112 (990)

Mori, F.; Okada, K-Ichi.; Nomura, T.; Kobayashi, Y., 2016: The Pedunculopontine Tegmental Nucleus as a Motor and Cognitive Interface between the Cerebellum and Basal Ganglia. NlmCategory="UNASSIGNED">As an important component of ascending activating systems, brainstem cholinergic neurons in the pedunculopontine tegmental nucleus (PPTg) are involved in the regulation of motor control (locomotion, posture an...

Mortimer Nick, 1996: Three- and four-pyroxene tholeiites from the Kirwans Dolerite, Reefton, New Zealand; a comparison with Ferrar and Tasmanian dolerites. International Gondwana Symposium 9, Vol

Fejza, A.; Schafia, A.; Löw, U.; Hille, K.; Seitz, B., 2013: Visual acuity and astigmatism after keratoplasty. Differences between the guided trephine system and motor trephine. The purpose of this study was to assess the differences in postoperative visual acuity, astigmatism, and selected postoperative complications between a guided trephine system (GTS) and motor trephine after penetrating keratoplasty. In this retrosp...