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Pigment extraction from lichens with dimethyl sulfoxide (DMSO) and estimation of chlorophyll degradation

, : Pigment extraction from lichens with dimethyl sulfoxide (DMSO) and estimation of chlorophyll degradation. Environmental and experimental botany 24(3): 239-245

Photosynthetic pigments from Romalina duriaei were extracted by immersion of the thalli in dimethyl sulfoxide (DMSO). Extraction was complete and the extract was found to be stable in the dark at 4°C for 10 days. The extract was measured spectrophotometrically at the blue and red region of the absorption spectrum and the ratio OD435/OD415 mm was found to be a preferable parameter for estimating chlorophyll degradation to phaeopigments. It is shown that Arnon's equation for quantification of total chlorophyll can be used with DMSO extracts too.


DOI: 10.1016/0098-8472(84)90004-2

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