Porcine pancreatic lipase immobilized on polysiloxane-polyvinyl alcohol hybrid matrix: catalytic properties and feasibility to mediate synthesis of surfactants and biodiesel
Paula, A.V.; Urioste, D.; Santos, J.C.; Castro, H.F. de, 2007: Porcine pancreatic lipase immobilized on polysiloxane-polyvinyl alcohol hybrid matrix: catalytic properties and feasibility to mediate synthesis of surfactants and biodiesel. Journal of Chemical Technology and Biotechnology 82(3): 281-288
Polysiloxane-polyvinyl alcohol hybrid matrix was prepared by a sol-gel technique and its capacity to bind porcine pancreatic lipase investigated. The loading of 250 units g-1 support was shown to be effective, resulting in an immobilized lipase with high catalytic activity. Both free and immobilized lipases were characterized by determining the activity profile as a function of pH, temperature, substrate concentration and thermal stability.DOI: 10.1002/jctb.1669
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