geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans


, : Ribosomal ribonucleic acid and ribosomal precursor ribonucleic acid in Anacystis nidulans. Biochemical Journal 129(1): 135-140

The RNA of the blue-green alga Anacystis nidulans contains three ribosomal RNA species with molecular weights of 0.56x10(6), 0.9x10(6), and 1.1x10(6) if the RNA is extracted in the absence of Mg(2+). The 0.9x10(6)mol.wt. rRNA is extremely slowly labelled in (32)P-incorporation experiments. This rRNA may be a cleavage product of the 1.1x10(6)mol.wt. rRNA from the ribosomes of cells in certain physiological states (e.g. light-deficiency during growth). The cleavage of the 1.1x10(6)mol.wt. rRNA during the extraction procedure can be prevented by the addition of 10mm-MgCl(2). (32)P-pulse-labelling studies demonstrate the rapid synthesis of two ribosomal precursor RNA species. One precursor RNA migrating slightly slower than the 1.1x10(6)mol.wt. rRNA appears much less stable than the other precursor RNA, which shows the electrophoretic behaviour of the 0.7x10(6)mol.wt. rRNA. Our observations support the close relationship between bacteria and blue-green algae also with respect to rRNA maturation. The conversion of the ribosomal precursor RNA species into 0.56x10(6)- and 1.1x10(6)-mol.wt. rRNA species requires Mg(2+) in the incubation medium.

US$19.90

PMID: 4630449

DOI: 10.1042/bj1290135


Other references

Ritchie A.W.S.; James K.; Chisholm G.D., 1984: The effect of anesthesia and surgery for benign disease of the upper urinary tract on circulating leukocyte subpopulations identified with monoclonal antibodies. Circulating lymphocyte subpopulations were monitored, using monoclonal antibodies and flow cytometry, in 6 patients undergoing surgery for benign disease of the urinary tract. A significant decrease in the total number of circulating lymphocytes w...

Jimenez Flores, R.; Ulibarri, A., 1995: Correlation between protein integrity and the quality of dairy products. Journal of Dairy Science 78(SUPPL 1): 145

Muthappa, B.N.; Kumari, K.N., 1977: Comparative efficacy of four fungicides for control of coffee rust in south India. A reprint of a paper on the control of Hemileia vastatrix already published elsewhere [RPP 56, 3570].

Guarino, C.; Nelson, M.; Dimenna, R., 1977: Operating Philadelphias northeast wastewater treatment plant. Progress in water technology: (6) 469-476

Bourvis, Nège.; Boelle, P-Yves.; Cesbron, J-Yves.; Valleron, A-Jacques., 2007: Risk assessment of transmission of sporadic Creutzfeldt-Jakob disease in endodontic practice in absence of adequate prion inactivation. Experimental results evidenced the infectious potential of the dental pulp of animals infected with transmissible spongiform encephalopathies (TSE). This route of iatrogenic transmission of sporadic Creutzfeldt-Jakob disease (sCJD) may exist in hu...

Yung, R.L.; Williams, R.M.; Richardson, B.C., 1995: Homing of autoreactive T cells in vivo. Journal of Investigative Medicine 43(SUPPL 3): 468A

Newman, M.E., 1991: Atlas maps cancer mortality among nonwhites. Journal of the National Cancer Institute 83(2): 89-89

Manouchehrifar, M.; Khosravi, S.; Khavanin, A.; Derakhshandeh, N., 2013: Report of 267 Cases of Scorpion Bite Referring to an Emergency Department during One Year. Scorpion bite is a common health problem in many parts of the world, including the Iran's tropics. There are thousands of cases and a number of deaths due to scorpion bite every year in the country. The present study aims to provide further d...

Zhang, Z-jie.; Hu, S-jiang.; Sun, J., 2007: Effects of atorvastatin on endothelium protection in spontaneously hypertensive rats. To investigate the effects of two different dosage of atorvastatin on endothelium protection in spontaneously hypertensive rats (SHR). SHRs (n=18) were randomly divided into three groups (n=6): SHR control group, 50 mg atorvastatin group and 10 mg...

Gould, D.J.; Macpherson, B., 2003: Evaluation of an undergraduate neuroscience research program at the university of kentucky. For the past 12 years the Department of Anatomy and Neurobiology at the University of Kentucky has run an interdisciplinary neuroscience research experience for undergraduates. Over the years the programs funding sources and participant numbers ha...